| Effects of isoflurane and ethanol on large conductance Ca^2＋ -activated K^＋ channels |
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| 引用本文： | 王英伟 熊源长 邓小明. Effects of isoflurane and ethanol on large conductance Ca^2＋ -activated K^＋ channels[J]. 中国人民解放军军医大学学报, 2004, 19(3): 181-182,186 |
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| 作者姓名： | 王英伟 熊源长 邓小明 |
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| 作者单位： | DepartmentofAnesthesiology,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai200433,China |
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| 摘 要： | To study the effect of isoflurane and ethanol on large conductance Ca^2 -activated K^ channels (BK channels). Methods: The cRNA of mslol encoding BK channels was injected into Xenopus oocytes. Oocytes were incubated in ND96 (96 mmol/L NaCI, 2.0 mmol/L KCI, 1.8 mmol/L CaCl2, 1.0 mmol/L MgCl2, and 5.0 mmol/L HEPES, pH 7.4) at 4 ℃. Patch clamp recording (outside-out) were performed after 2-3 d. Isoflurane was administrated by the vaporizer driven by air, ethanol was applied by a cloud, manual-controlled administration system. Different test potentials from 0 to 10 mV were given to observe changes of currents. Results: 0.7 mmol/L and 1.2 mmol/L of isoflurane could inhibit BK currents obviously at different command potentials, but 50 mmol/L, 100 mmol/L, or 200 nunol/L of ethanol had no any effect on BK currents. Conclusion: Clinical concentration of isoflurane can distinctly inhibit isolating BK currents.
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| 关 键 词： | 异氟烷 酒精 Ca^2＋活性 K^＋通道 电导系数 |
Effects of isoflurane and ethanol on large conductance Ca2+ -activated K+ channels |
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| WANG Ying-wei,XIONG Yuan-chang,DENG Xiao-ming. Effects of isoflurane and ethanol on large conductance Ca2+ -activated K+ channels[J]. Journal of Medical Colleges of PLA(China), 2004, 19(3): 181-182,186 |
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| Authors: | WANG Ying-wei XIONG Yuan-chang DENG Xiao-ming |
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| Affiliation: | WANG Ying-wei,XIONG Yuan-chang,DENG Xiao-ming Department of Anesthesiology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | |
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| Keywords: | isoflurane ethanol patch clamp large conductance Ca 2+-activated K + channels |
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