线粒体DNA异质性检测方法研究进展

线粒体是真核细胞内的重要细胞器,能量生成的场所,参与脂肪酸的合成及某些蛋白质的合成,也是核外惟一的遗传物质。线粒体在生物的生长、发育、代谢、衰老、疾病、死亡以及生物进化等方面都有非常重要的意义。由于线粒体DNA具有母系遗传、拷贝数多、阈值效应、较高的突变率和序列的多样性等特点,因此线粒体DNA异质性在生物学、医学、法医、生物技术等领域有重要的应用。近几年来,mtDNA异质性研究的新成就为揭示许多疾病的发生机制提供了新的线索。现就线粒体DNA异质性检测方法及研究等进行综述。

Study Progress about Detection Method of Heteroplasmy within Mitochondrial DNA

Mitochondria are essential organelles in eukaryotic cells,and are the site of energy generation.They are involved in the synthesis of fatty acids and other proteins,they are also the only genetic material outside of the nuclear.Mitochondria have important significance in growth,development,metabolism,aging,disease,death and biological evolution.Because mitochondria have many characteristics such as maternal inheritance,large number of copies,threshold effect,higher mutation rate and sequence diversity,the h...