1例疑似HIV抗体阳性样本的检测分析

目的对1例艾滋病病毒（HIV）抗体确证试验疑似阳性样本进行随访检测分析,探讨最新《全国艾滋病检测技术规范》（2009年修订版）在实际工作中可能存在的问题及今后的解决思路。方法对蛋白印迹试验（WB）条带为gp160、gp120、p24的患者进行跟踪随访,按常规检测程序进行HIV抗体筛查和确证试验,采集的血液标本同时补充检测HIV核酸。结果 5次WB试验带型均为gp160、gp120、p24,4次病毒核酸随访检测均未检出HIV核糖核酸（RNA）和脱氧核糖核酸（DNA）,最终判断为无HIV感染。结论对刚符合阳性判定标准的样本应予高度警惕,切勿急于出具确证阳性报告,应补充检测病毒核酸,并继续随访检测,观察条带是否有进展

Detection of one suspected positive case by HIV-AB test

Objective To explore the potential problems and future solutions in National AIDS Testing Technology Standards（revised edition 2009）applied in practical work by following up one suspected positive case by HIV（Human immunodeficiency virus）-AB confirmation test. Methods The case with WB（Western blotting test） belts for gp160, gpl20 and p24 was followed up and both screening and confirmation tests were carried out according to a routine procedure; the collected blood sample was additionally tested for HIV nucleic acid. Results Of the 5 tests, the type of WB belts were gpl60, gpl20 and p24 respectively. No HIV RNA（ribonucleic acid） or DNA（deoxyribonucleic acid）were detected in the four follow-up nucleic acid tests. So the case was finally confirmed as being free of HIV infection. Conclusion The sample which barely satisfies positive criterion should be taken with caution, and a final report of HIV positivity should not be made in a rash. Instead, virus nucleic acid testing should be added and follow-up should be continued to look at the progress of WB belt.