| 去氢骆驼蓬碱诱导人胃腺癌SGC-7901细胞凋亡 |
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| 引用本文： | 宋震亚,刘济仁,陆新良,王良静. 去氢骆驼蓬碱诱导人胃腺癌SGC-7901细胞凋亡[J]. 中药材, 2006, 29(6): 571-573 |
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| 作者姓名： | 宋震亚 刘济仁 陆新良 王良静 |
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| 作者单位： | 浙江大学医学院附属第二医院消化科,浙江杭州310009 |
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| 摘 要： | 目的：探讨去氢骆驼蓬碱（Harmine，HM）诱导人胃腺癌SGC-7901细胞凋亡的作用。方法：MTT法检测HM对该细胞系的生存抑制率，光镜下观察凋亡细胞，流式细胞仪（FCM）检测凋亡率，基因组DNA琼脂糖凝胶电泳（DNALadder）检测凋亡梯状条带。结果：HM处理SGC-7901细胞后，细胞的存活率明显降低；细胞形态观察到有核固缩与核断裂的凋亡现象；流式细胞仪测定细胞周期的G1期前有亚二倍体的凋亡峰；基因组DNA琼脂糖凝胶电泳检测到明显的凋亡梯状条带。结论：HM能诱导人胃腺癌SGC-7901细胞的凋亡。
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| 关 键 词： | 去氢骆驼蓬碱 人胃腺癌SGC-7901细胞 细胞凋亡 |
| 文章编号： | 1001-4454(2006)06-0571-03 |
| 收稿时间： | 2005-11-24 |
| 修稿时间： | 2005-11-24 |
Harmine Induces Apoptosis in Human SGC-7901 Cells |
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| SONG Zhen-ya,LIU Ji-ren,LU Xin-liang,WANG Liang-jing. Harmine Induces Apoptosis in Human SGC-7901 Cells[J]. Journal of Chinese medicinal materials, 2006, 29(6): 571-573 |
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| Authors: | SONG Zhen-ya LIU Ji-ren LU Xin-liang WANG Liang-jing |
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| Affiliation: | Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009 ,China |
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| Abstract: | Objectvie: To investigate the effect of apoptosis induced in human SGC-7901 cells by Harmine. Methods: The effect of Harmine on human SGC-7901 cell survival and apoptosis was determined by MTT assay, light microscopy and flow cytometry. Cell genomic DNA was detected by agarose electrophoresis. Results: The survival of human SGC-7901 cells decreased; Apoptotic cells were observed by fluorescent microscope; FCM analysis showed that the peak of apoptosis increased. Typical DNA Ladder were detected in DNA agarose gel electrophoresis. Conclusion: HM can induce apoptosis in human SGC-7901 cells. |
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| Keywords: | Harmine SGC-7901 Apoptosis |
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