Dlx5, the mouse homologue of the human-imprinted DLX5 gene,is biallelically expressed in the mouse brain |
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Authors: | Motoshi?I?Kimura Yasuhiro?Kazuki Akiko?Kashiwagi Yoshiteru?Kai Satoshi?Abe Ottavia?Barbieri Giovanni?Levi Email author" target="_blank">Mitsuo?OshimuraEmail author |
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Institution: | (1) Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishimachi, Yonago 683-8503, Japan;(2) Laboratory Animal Research Center, Faculty of Medicine, Tottori University, Yonago, Japan;(3) Transgenic Unit, Department of Oncology, Biology and Genetic, University of Genova, IST c/o CBA, L.go Rosanna Benzi 10, 16132 Genova, Italy;(4) CNRS UMR5166-MNHN, Evolution des Regulations Endocriniennes, 7, rue Cuvier, 75231 Paris, Cedex 05, France |
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Abstract: | The mouse Dlx5 gene encodes a distal-less-related DNA-binding homeobox protein first expressed during early embryonic development in anterior regions of mouse embryo and is located on chromosome 6, which is the syntenic region to the human chromosome 7q21–q31 imprinting cluster. Recently, its human homologue, DLX5, was identified to be imprinted and maternally expressed, at least in normal human lymphoblasts and in brain tissues. In our study, we analyzed the imprinting status of mouse Dlx5 by RT-PCR, first in the F1 of a reciprocal cross between two different mouse strains, and second in heterozygous Dlx5 mutant mice. Both approaches revealed that mouse Dlx5 followed a biallelic pattern of expression in brain tissue and in testis. Our findings suggest that the Dlx5 gene escapes genomic imprinting, at least in mice of certain genetic backgrounds. |
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Keywords: | DLX5 Dlx5 Imprinting Brain Mouse chromosome 6 Maternally expressed gene |
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