| 大鼠切牙颈环上皮细胞的培养 |
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| 引用本文： | 刘晓辉,苑芳,方军,金岩,文玲英. 大鼠切牙颈环上皮细胞的培养[J]. 临床口腔医学杂志, 2009, 25(5): 273-275 |
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| 作者姓名： | 刘晓辉 苑芳 方军 金岩 文玲英 |
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| 作者单位： | 解放军兰州军区总医院口腔科,甘肃,兰州,730050;解放军第一医院;第四军医大学口腔医学院 |
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| 摘 要： | 目的：培养大鼠切牙颈环上皮细胞。方法：选择出生后（postnatal，PN）8d的SD大鼠，分离下颌切牙牙胚，切取切牙颈环结构，酶消化法细胞培养。通过差别消化法初步纯化颈环上皮细胞，抗角蛋白免疫化学染色初步鉴定颈环上皮细胞。结果：原代培养细胞为上皮和间充质混杂的细胞，经初步纯化获得铺路石样排列的，角蛋白阳性的颈环上皮细胞团。结论：实验成功培养了大鼠切牙颈环上皮细胞。
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| 关 键 词： | 大鼠 切牙 颈环 上皮细胞 |
Culture of rat incisor cervical-loop epithelial cell |
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| LIU Xia-ohui,YUAN Fang,FANG Jun,JIN Yan,WEN Ling-ying. Culture of rat incisor cervical-loop epithelial cell[J]. Journal of Clinical Stomatology, 2009, 25(5): 273-275 |
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| Authors: | LIU Xia-ohui YUAN Fang FANG Jun JIN Yan WEN Ling-ying |
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| Affiliation: | LIU Xiao-hui,YUAN Fang,FANG Jun,JIN Yan,WEN Ling- ying. Department of Stomatology,the General Hospital of Lanzhou Comm,,Gansu Lanzhou 730050,China. |
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| Abstract: | Objective:To culture rat incisor cervical-loop epithelial cells. Method:Mandibular incisor germs were isolated from PN8d SD rat. The cervical-loop structure of incisor germ was cut and digested by enzyme for cell culture. Cervical-loop epithelial cells were preliminarily purified by differential digestion and identified by immunostaining with antikeratin antibody. Result:Primary cells were heterogenous,consisting of epithelial and mesenchymal cells. Cervical-loop epithelial cells were cobblestone-shaped and... |
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| Keywords: | rat incisor cervical-loop epithelial cell |
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