| 鱼腥草多糖对小鼠肝、肾、心肌和脑组织抗氧化作用的研究 |
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| 引用本文： | 刘光建,王璐,王菲菲,王彩红,孙设宗. 鱼腥草多糖对小鼠肝、肾、心肌和脑组织抗氧化作用的研究[J]. 中国实验方剂学杂志, 2011, 17(8): 207-210 |
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| 作者姓名： | 刘光建 王璐 王菲菲 王彩红 孙设宗 |
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| 作者单位： | 1. 湖北医药学院第一临床学院麻醉学系,湖北,十堰,442000
2. 湖北医药学院生化与免疫学实验室,湖北,十堰,442000 |
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| 摘 要： | 目的:探讨鱼腥草多糖对小鼠肝、肾、心和脑组织体内外的抗氧化作用。方法:昆明种小鼠50只随机分空白对照组、病理模型组;鱼腥草多糖各保护组,每天分别给30,60,120 mg·kg-1剂量的鱼腥草多糖ig,连续7 d,末次ig 2 h,空白对照组腹腔ip调和油溶液,其余各组ip 0.15%CCl4调和油溶液,24 h眼球取血测定血清中ALT,AST活性。处死小鼠取出肝脏称质量计算肝体指数;制备肝、肾、心、脑组织匀浆测定MDA的含量,经fenton反应诱导产生自由基检测各组织,吸光度(A532 nm)值。结果:鱼腥草多糖可减轻实验性肝损伤所致炎性浸润,降低肝体指数,降低血清中ALT,AST活性(P<0.05)和各组织MDA的含量(P<0.01);体外抗氧化实验表明鱼腥草多糖能抑制脂质过氧化物的生成,A532 nm明显降低(P<0.01)。结论:鱼腥草多糖能降低CCl4损伤小鼠血清AST,ALT活性,降低各组织MDA的含量,对小鼠肝、肾、心肌、脑组织体内外有抗氧化作用,尤其对肝、肾抗氧化作用较强。
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| 关 键 词： | 鱼腥草多糖 自由基 抗氧化 |
| 收稿时间： | 2010-11-07 |
Anti-oxidant Effect of Houttuyniae Herba Polysaccharide on Mouse Liver, Kindey, Heart and Brain Tissues |
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| LIU Guang-jian,WANG Lu,WANG Fei-fei,WANG Cai-hong and SUN She-zong. Anti-oxidant Effect of Houttuyniae Herba Polysaccharide on Mouse Liver, Kindey, Heart and Brain Tissues[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(8): 207-210 |
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| Authors: | LIU Guang-jian WANG Lu WANG Fei-fei WANG Cai-hong SUN She-zong |
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| Affiliation: | Department of Anesthesiology, First Clinical College, Hubei Medical University, Shiyan 442000, China;Department of Anesthesiology, First Clinical College, Hubei Medical University, Shiyan 442000, China;Department of Anesthesiology, First Clinical College, Hubei Medical University, Shiyan 442000, China;Department of Anesthesiology, First Clinical College, Hubei Medical University, Shiyan 442000, China;Biochemistry and Immunology Laboratory, Hubei Medical University, Shiyan 442000, China |
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| Abstract: | Objective:To investigate the anti-oxidant effect in vitro and in vivo of Houttuyniae Herba polysaccharide on mouse liver,kidney,and heart and brain tissues.Method:Kunming mice were divided into model group,control group and Houttuyniae Herba polysaccharide group.Three Houttuyniae Herba Polysaccharide subgroups were given Houttuyniae Herba polysaccharide for 7 days with doses of 30,60,120 mg.kg-1respectively.The control group was ip injected blend oil,and the other groups was ip given 0.15% CCl4 mixed with b... |
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| Keywords: | Houttuyniae Herba polysaccharide free radicals antioxidation |
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