| 不同干燥方法对祛湿化瘀方 防治实验性脂肪肝药效学的影响 |
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| 引用本文： | 冯琴,张钰泉,张宁,李红山,李琦,许丽莉,彭景华,刘平,胡义扬. 不同干燥方法对祛湿化瘀方 防治实验性脂肪肝药效学的影响[J]. 中国实验方剂学杂志, 2011, 17(9): 137-141 |
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| 作者姓名： | 冯琴 张钰泉 张宁 李红山 李琦 许丽莉 彭景华 刘平 胡义扬 |
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| 作者单位： | 1. 上海中医药大学附属曙光医院,肝肾疾病证候教育部重点研究室,上海市高校中医内科学E-研究院,上海,201203
2. 上海中医药大学科技实验中心,上海,201203 |
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| 基金项目： | 国家自然科学基金面上项目，国家自然基金青年基金项目，上海市教委创新团队项目 |
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| 摘 要： | 目的:探讨不同干燥方法对"祛湿化瘀方"防治实验性脂肪肝药效学的影响。方法:祛湿化瘀方中5味药物分别醇提、水提醇沉后合并,制备成生药含量为0.93 g.mL-1的药液,再按不同干燥方法制得浸膏、70℃真空负压干燥药物、40℃真空负压干燥药物、冷冻干燥药物、喷雾干燥药物。采用Wistar雄性大鼠,以四氯化碳(CCl4)复合高脂低蛋白饮食制备大鼠脂肪肝模型,造模2周后,将造模大鼠随机分为模型组、未干燥组、浸膏组、70℃真空负压干燥组、40℃真空负压干燥组、冷冻干燥组、喷雾干燥组,每组灌以相应药物2周。取材后检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转移酶(GGT)活性、肝组织甘油三酯(TG)、游离脂肪酸(FFA)的含量变化。并对各个药物样品进行指纹图谱分析。结果:与正常组相比,模型组ALT,AST,GGT活性,肝组织TG,FFA含量显著升高。未干燥组与模型组相比,血清ALT,AST,GGT及肝组织TG,FFA显著下降;浸膏组与模型组相比,血清GGT,ALT,肝组织TG,FFA显著下降;70℃和40℃真空干燥组除血清ALT活性显著降低外,其余指标与模型组相比均无显著差异;冷冻干燥组除肝组织TG显著低于模型组,其余指标与模型组相比均无显著差异;喷雾干燥组除血清GGT显著低于模型组,其余指标与模型组相比均无显著差异;未干燥组的综合疗效优于其他各组。运用指纹图谱对各组药物进行分析,色谱图有相应的谱峰信息综合表达有差异。结论:"袪湿化瘀方"经不同干燥方法后对实验性脂肪肝药效均有所下降,不同方法干燥后部分有效物质丢失,可能是导致药效差异的主要原因。
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| 关 键 词： | 祛湿化瘀方 干燥方法 实验性脂肪肝 药效学 指纹图谱分析 |
| 收稿时间： | 2011-01-19 |
Effects of Different Drying Methods of Qushi Huayu Decoction on Pharmacodynamics of Experimental Fatty Liver |
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| FENG Qin,ZHANG Yu-quan,ZHANG Ning,LI Hong-shan,LI Qi,XU Li-li,PENG Jing-hu,LIU Ping and HU Yi-yang. Effects of Different Drying Methods of Qushi Huayu Decoction on Pharmacodynamics of Experimental Fatty Liver[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(9): 137-141 |
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| Authors: | FENG Qin ZHANG Yu-quan ZHANG Ning LI Hong-shan LI Qi XU Li-li PENG Jing-hu LIU Ping HU Yi-yang |
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| Affiliation: | Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Experimental Centre for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Experimental Centre for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboroatory Approved by Educatiojn Ministry for Syndromes of Liver and Kidney Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | Qushi Huayu decoction drying methods experimental fatty liver pharmacodynamics fingerprint analysis |
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