多指标综合评价南五味子木脂素提取工艺

目的:筛选出提取南五味子总木脂素的最佳工艺。方法:采用紫外分光光度法和HPLC法相结合对南五味子总木脂素进行检测,以回流提取方法,采用正交试验设计,以提取时间、乙醇体积分数、溶媒用量、提取次数为因素,分别以总木脂素、五味子甲素、五味子酯甲为评价指标,确定最佳提取条件;结果:用55%乙醇,溶媒用量为16倍,提取时间为60 min,提取次数为2次效果最好。结论:采用多指标综合评价法能够更客观优化南五味子总木脂素工艺条件的提取工艺,为南五味子总木脂素分离纯化研究提供参考。

Multi-index Evaluation of Extraction Process of Total Lignans from Schisandra sphenanthera

Objective: To optimize the extract process of total lignans from Schisandra sphenanthera. Method: The best extraction conditions were determined by ultraviolet spectrophotometry method and HPLC method based on the test of total lignans from S.sphenanthera; and the orthogonal experiment design was used with a total lignans, fructus schisandrae chinensis and schizandrol A as evaluation indexes, using reflux extraction method with extraction time, ethanol concentration, solvent dosage, extraction times as factors. Result: The optimal extracting process was as follows: 16 times of 55% ethanol solvent consumption, extracted for 60min with 2 times. Conclusion: The extraction process of Schisandra sphenanthera objectively optimized by multi-index synthetic evaluation method can provide the reference for the purification research of total lignans from S. sphenanthera.