黑布药膏对兔耳增生性瘢痕成纤维细胞增殖与凋亡的影响

目的:探讨黑布药膏对兔耳增生性瘢痕成纤维细胞增殖与凋亡的影响。方法:成年大耳白兔24只,建立兔耳增生性瘢痕动物模型,21 d后,将瘢痕动物模型随机分为黑布药膏治疗组和瘢痕模型组,在黑布药膏治疗组瘢痕局部涂抹黑布药膏,每3 d 1次,连续用药56 d。在用药第2,4,6,8周分别切取两组瘢痕组织,对比研究在瘢痕形成过程中黑布药膏对兔耳瘢痕成纤维细胞的影响,采用免疫组化方法检测增殖细胞核抗原(PCNA)蛋白表达和细胞凋亡的原位检测。结果:按不同时间段取材进行免疫组化染色,高倍镜下观察,结果显示:黑布药膏治疗组和模型对照组比较,PCNA蛋白表达明显减弱(P<0.05),细胞凋亡增加(P<0.05)。结论:黑布药膏可抑制兔耳增生性瘢痕成纤维细胞的增殖过程,诱导细胞凋亡。黑布药膏可抑制兔耳增生性瘢痕组织增生。

Effect of Heibu Ointment on Proliferative Activity and Apoptosis of Fibroblats in Hypertrophic Scarring Model in Rabbit Ears

Objective: To investigate the effect of Heibu Ointment on proliferative activity and apoptosis of fibroblasts in the hypetrrophic scarring model in rabbit ears. Method: Twenty-four white rabbits were used to establish the model of hypertrophic scar in ears.After twenty-one days, the model rabbits were randomly divided into Heibu Ointment treatment group and model group. In Heibu Ointment treatment group, Heibu Ointment was plastered on the hypetrrophic scars once in every three days. The treatment course lasted for fifty-six days. The scar tissue was sampled on the second, the fourth, the sixth and the eighth week for the two groups. The changes in fibroblasts treated with Heibu Ointment were compared with that of the controls during the forming of hypertrophic scars. The expression of proliferation cell nuclear antigen (PCNA) was measured by immunohistochemistry technique, and the apoptosis was measured by TUNEL. Result: The expression of PCNA in the Heibu Ointment treatment group was obviously weaker than that of the controls (P<0.05),and the apoptosis was increased in Heibu Ointment treatment group (P<0.05). Conclusion: Heibu Ointment can inhibit the proliferation of fibroblasts and accelerate cell apoptosis in hypertrophic scars in rabbit ears.