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| 大黄酸对Caco-2细胞多药耐药蛋白转运体表达的影响 | |
| 引用本文： | 杨娜,隋峰,李沧海,李兰芳,张畅斌,郭淑英,朱晓新,姜廷良. 大黄酸对Caco-2细胞多药耐药蛋白转运体表达的影响[J]. 中国实验方剂学杂志, 2011, 17(9): 133-136 |
| 作者姓名： | 杨娜 隋峰 李沧海 李兰芳 张畅斌 郭淑英 朱晓新 姜廷良 |
| 作者单位： | 1. 首都医科大学中医药学院,北京,100069 2. 中国中医科学院中药研究所唐氏中药研究中心,北京,100700 3. 南京中医药大学,南京,210046 4. 首都医科大学中医药学院,北京,100069;中国中医科学院中药研究所唐氏中药研究中心,北京,100700 |
| 基金项目： | 国家"十一五"科技重大专项综合性中药新药研究开发技术大平台 |
| 摘 要： | 目的:探讨大黄酸对人结肠腺癌上皮细胞(Caco-2)多药耐药蛋白mRNA表达的影响。方法:用大黄酸作用于Caco-2细胞,采用荧光定量PCR(real time PCR)法检测转运体MRP1-5 mRNA的表达量,数据分析采用2-ΔΔCT法。结果:高(5.00 mg.L-1)低(2.50 mg.L-1)质量浓度大黄酸均可以显著上调Caco-2细胞MRP3 mRNA表达(P<0.05或P<0.01);低浓度大黄酸可以显著下调Caco-2细胞MRP5 mRNA表达(P<0.05);大黄酸对MRP1,MRP2和MRP4 mRNA的表达没有显著影响。结论:大黄酸可能通过上调Caco-2细胞MRP3 mRNA的表达和下调MRP5 mRNA的表达而影响其口服药物的生物利用度。 |
| 关 键 词： | Caco-2细胞 大黄酸 荧光定量聚合酶链反应 多药耐药蛋白 |
| 收稿时间： | 2011-01-30 |
Effects of Rhein on Expression of Multi-drug Resistance Protein in Caco-2 Cells |
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| YANG N,SUI Feng,LI Cang-hai,LI Lan-fang,ZHANG Chang-bin,GUO Shu-ying,ZHU Xiao-xin and JIANG Ting-liang. Effects of Rhein on Expression of Multi-drug Resistance Protein in Caco-2 Cells[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(9): 133-136 | |
| Authors: | YANG N SUI Feng LI Cang-hai LI Lan-fang ZHANG Chang-bin GUO Shu-ying ZHU Xiao-xin JIANG Ting-liang |
| Affiliation: | The School of Chinese Medicine, Capital Medical University, Beijing 100069, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Nanjing University of Chinese Medicine, Nanjing 210046, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;The School of Chinese Medicine, Capital Medical University, Beijing 100069, China; Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | |
| Keywords: | Caco-2 cell rhein real-time PCR multi-drug resistance protein |
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