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目的探讨氟斑牙的发病机制及褪黑素是否对氟斑牙的发生有拮抗作用。方法 2009年3—10月于中国医科大学公共卫生学院将40只Wistar大鼠随机分为6组,包括阴性对照组、低氟组、高氟组、低氟+褪黑素组、高氟+褪黑素组。建立氟斑牙动物模型,制作切片后行HE染色,光镜下观察各组大鼠切牙成釉细胞形态。结果单纯给氟组大鼠。出现牙面粗糙、棕白色相间横纹、白垩色改变,高氟组大鼠的氟斑牙改变较低氟组的改变典型;成釉细胞扭曲变形,正常的高柱状形态丧失,胞内出现空泡等。注射褪黑素组与单纯给氟组的切牙一般状态及成釉细胞形态、排列未见明显差异。结论氟对大鼠切牙的成釉细胞有毒性效应,饮水氟含量高所致氟斑牙症状加重。褪黑素对氟斑牙的发生是否有拮抗作用有待进一步研究。

The effect of fluoride on the morphology of ameloblast in rat incisor and the antagonistic effect of melatonin

Objective To explore the formation mechanism of dental fluorosis and infer whether melatonin have antagonism to dental fluorosis.Methods From March to July of 2009,40 Wistar rats were randomly divided into 6 groups,and the animal models of fluorisis were established.HE was used to observe the changes of the ameloblasts in rat incisors.Results The surface of the rat teeth fed with fluoride appeared chalky color changes and the cross striations could be seen on the enamel surface.The ameloblast showed disarrangement,cells could be arranged in multi-layer,and even had vacuolar change.The change of the ameloblast in the melatonin group and the fluoride group had no significant difference.Conclusion Fluoride has a toxic effect on the ameloblast of the rat incisor.The antagonistic effect of the melatonin on the dental fluorosis is not definite.