| 伯氏疟原虫ANKA株染色体核型分析 |
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| 引用本文： | 陈颖丹,张家埙,林宝英. 伯氏疟原虫ANKA株染色体核型分析[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(3): 176-178 |
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| 作者姓名： | 陈颖丹 张家埙 林宝英 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾,血吸虫病合作中心, |
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| 基金项目： | 国家自然科学基金资助课题(No.39770671) |
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| 摘 要： | 目的 分析伯氏疟原虫 ANKA株染色体分子核型 ,确定染色体的数目与大小。 方法 用脉冲凝胶电泳 (PFGE)方法对伯氏疟原虫 ANKA株进行核型分析。 结果与结论 伯氏疟原虫 ANKA株染色体共 14条 ,其大小为 0 .6～ 3 Mb。第 5～ 7和第 9～ 12条染色体在电泳中表现为共迁移。为利用多种特异性基因制作探针进行杂交 ,并对特异基因进行染色体定位提供参考。
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| 关 键 词： | 脉冲凝胶电泳 伯氏疟原虫ANKA株 染色体核型 |
| 文章编号： | 1000-7423(2001)-03-0176-03 |
| 修稿时间： | 2000-11-28 |
Analysis on Karyotypes of ANKA strain of Plasmodium berghei |
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| CHEN Ying-dan,ZHANG Jia-xun,LING Bao-ying. Analysis on Karyotypes of ANKA strain of Plasmodium berghei[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2001, 19(3): 176-178 |
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| Authors: | CHEN Ying-dan ZHANG Jia-xun LING Bao-ying |
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| Affiliation: | Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025. |
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| Abstract: | OBJECTIVE: To analyze the molecular karyotypes of ANKA strain of Plasmodium berghei and demonstrate the size and number of chromosomes. METHODS: To isolate the genome DNA of P. berghei ANKA strain and analyze molecular karyotypes through CHEF-III pulsed field gel electrophoresis (PFGE). RESULTS: The number of chromosomes was found to be 14, and their size ranged from 0.6 Mb to 3 Mb. Chromosomes number 5 to 7 and 9 to 12 appeared co-migrated in the gel. CONCLUSION: PFGE technique is useful for analyzing the molecular karyotypes and may be also useful for further study to locate the special gene on chromosomes and carry out the genetic characters and mechanism of drug resistance. |
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| Keywords: | pulsed field gel electrophoresis ANKA strain of Plasmodium berghei molecular karyotypes |
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