人参总皂甙对大鼠烟雾吸入性肺损伤的疗效与其抗氧化作用的关系

本实验采用大鼠烟雾吸入性肺损伤模型,观察了致伤后立即腹腔注射人参总皂甙(GS)对该损伤的作用。发现GS可明显减轻烟雾吸入所致PMVP增加,减少BALF中WBC计数和蛋白含量,使肺内WBC浸润,肺水肿,肺充血、出血等病变减轻。GS还可抑制致伤大鼠肺和血清MDA的升高,使已降低了的肺SOD活性恢复至正常水平以上。体外实验也发现,GS可有效地抑制肺匀浆过氧化脂质生成和清除O_2~-·、OH·。提示GS的治疗效果与其抗氧化作用有关。

The Relationship between the Antioxidation of Ginsenoside and Effects on Smoke Inhalation Injury in Rats

The smoke inhalation injury of lung of rats model is used to investigate the effects of ginsenosides(GS). GS alleviates the pulmonary microvascular permeability (PMVP), reduces the protein and leukocytes(WBC) in bronchoalveoli lavage fluid(BALF)in the inhalation injuried rats. Vascular WBC accumulation, interstitial WBC infiltration, interstitial and intra-alveolar edema, hemorrhage and vascular congestion by histopathological examination are reduced distinctly by GS. GS also inhibits the elevation of malondialdehyde (MDA) in lung and serum and revers the increased activity of superoxide dismutase in lung after smoke inhalation. The experiments in vitro also show the inhibition of lipid peroxida- tion of lung homogenate, and scavenging of superoxide anion and hydroxyl radical effectively in proper doses. These results imply a close relationship between the efficacy of GS treatment for lung inhalation injury and its antioxidation.