四逆汤对缺血-再灌注离体鼠肺的保护作用

目的： 建立离体大鼠肺灌流模型，研究四逆汤（SND）对缺血-再灌注肺的保护作用及可能机制。方法：将SD大鼠随机分成假手术组、模型组和模型+SND组，观察SND对大鼠肺组织形态学改变、平均肺动脉压（MPAP）、肺组织湿/干重比（W/D）、灌流液及肺组织匀浆中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量以及肺组织匀浆中一氧化氮（NO）含量和一氧化氮合酶（NOS）活力的影响。结果：模型+SND组的肺泡壁增厚程度和肺泡水肿程度显著低于模型组，肺组织W/D值及MPAP显著小于模型组，灌流液和肺组织匀浆中SOD活性显著高于模型组，而MDA含量显著低于模型组。SND可显著抑制缺血-再灌注引起的肺组织NO含量的减少。但3组间比较，NOS含量无显著差异。结论：SND有抗大鼠肺缺血-再灌注损伤作用，其机制可能与清除氧自由基和改善组织灌流有关。

Effects of Sini decoction against pulmonary injury induced by ex vivo ischemia-reperfusion in rats

AIM: To observe the effects of Sini decoction against pulmonary injury induced by ex vivo ischemia-reperfusion in rats. METHODS: The model of ischemia-reperfusion was established. Twenty-four Sprague-Dawley rats were randomly divided into Sham, I/R, and SND groups. Wet to dry lung weight ratio (W/D), mean pulmonary artery pressure (MPAP), SOD activity and MDA contents in pulmonary perfusate and tissue, NOS activity and NO contents in pulmonary tissue were detected. The pathologic changes in pulmonary tissue were also observed by light microscope. RESULTS: The morphological changes of pulmonary injury were alleviated in SND group. Wet/dry ratio, MPAP and MDA contents in pulmonary perfusate and tissue were significantly lower in SND group after ischemic/reperfusion. SOD activity in pulmonary perfusate and tissue, and NO contents in pulmonary tissue were significantly higher in SND group than those in I/R group. No significant difference in NOS activity in pulmonary tissue among three groups was observed. CONCLUSION: These results indicate that SND may have a protective effect on ischemia-reperfusion injured lung by its antioxidant activity and by adjusting NO level.