抵抗素、miRNA-26b在婴幼儿毛细支气管炎中的表达

目的 探讨婴幼儿毛细支气管炎中miRNA-26b、抵抗素的变化.方法 收集毛细支气管炎患儿的血液,以正常儿童为对照,通过酶联免疫吸附(ELISA)法测定血清中白介素(IL)-8、抵抗素浓度,并利用实时定量PCR技术测定血液淋巴细胞中miRNA-26b、抵抗素mRNA表达,利用miRNA-26b mimics干预人肺上皮细胞A549,通过实时定量PCR技术测定miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度变化.结果 毛细支气管炎患儿外周血清中IL-8、抵抗素浓度均升高,血液淋巴细胞中miRNA-26b、抵抗素mRNA表达增加;经过干预的A549细胞miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度均有增加.结论 miRNA-26b、抵抗素在毛细支气管炎患儿血液淋巴细胞中存在差异表达,而这可能参与了炎症反应的发生.

Expressions of resistin and miRNA-26b in bronchiolitis in infants

Objective To study the expression and slinical significance of miRNA-26b, resistin and inflammatory mediator in bronchiolitis.Methods Serum IL-8, resistin concentration(by enzyme linked immunosorbent assay, ELISA), microRNA(miRNA)-26b with resistin mRNA expression in blood lymphocytes(by real-time quantitative PCR assay) were analyzed in children with bronchiolitis and healthy age-match control.After miRNA-26b mimics treating human lung epithelial cells A549, IL-8 concentrations in cell supernatants, miRNA-26b and resistin in cells were analyzed.Results In children with bronchiolitis, serum IL-8, resistin protein concentrations were increaced, as well as miRNA-26b and resistin mRNA in blood lymphocytes.After the intervention of A549 cells miRNA-26b, resistin mRNA and IL-8 protein concentrations in supernatants were increased.Conclusion miRNA-26b and resistin expression change in blood lymphocytes in children with bronchiolitis, which may be involved in the inflammatory response.