硫化氢对不同周龄自发性高血压大鼠脑基底动脉舒张反应的影响

目的 探讨硫化氢(H2S)对不同周龄自发性高血压大鼠(SHR)离体脑基底动脉舒张反应的影响.方法 选取14、40周龄SHR和正常Wistar京都(WKY)大鼠各6只,测量血压后,急性分离脑基底动脉,应用压力肌动图技术检测不同浓度硫氢化钠(NaHS)对大鼠脑基底动脉直径变化的影响.结果 ① 10、30、100、300、1 000、3 000 mmol/L的NaHS能够浓度依赖地舒张SHR和WKY大鼠脑基底动脉;② 与14周龄的WKY相比,1 000、3 000 mmol/L的NaHS对14周龄 SHR的舒张率更明显,两者间有显著性差异(P<0.05);③ 与40周龄的WKY大鼠相比,1 000、3 000 mol/L的NaHS对40周龄 SHR的舒张率降低,两者间有显著性差异(P<0.05).结论 在高血压初期,脑基底动脉对H2S的敏感性增加,而随着高血压的发展及血管功能的严重损伤,脑基底动脉可能对H2S的敏感性降低.

Vasodilator effect of hydrogen sulfide on basilar artery in spontaneously hypertensive rats of different weeks

Objective To investigate the vasodilator effect of hydrogen sulfide (H2S) on basilar artery in spontaneously hypertensive rats(SHR) with different age.Methods 14-and 40-weeks old SHR and Wistar-Kyoto (WKY) rats were used to measure the blood pressure,and their cerebral basilar artery was acutely dissociated.Pressure myography was used to examine the differences for the vasodilator effect of H2S donor NaHS with different concentration on basilar artery between SHR and normotensive WKY rats.Results Our results showed that ① NaHS of 10,30,100,300,1 000,3 000 mmol/L could relax the cerebral basilar artery in SHR and WKY rats;② Compared with 14-and 40-weeks old WKY,after applying 1 000,3 000 mmol/L NaHS,the effect of NaHS-induced vasodilator was greater in age-matched SHR (P<0.05);③ Compared with 14 weeks of SHR,NaHS-induced vasodilator effect in cerebral basilar artery of 40-weeks old SHR was less obvious (P<0.05).Conclusion Cerebral basilar artery of SHR exhibits enhannced sensitivity to H2S induced vasodilatation during early stage of hypertension,however,pathological development of hypertension and serious injury in vascular function may lead to reduce H2S sensitivity in cerebral basilar artery of SHR.