RNAi沉默PCDGF基因对喉鳞癌Hep-2细胞生物学特性的影响

目的 研究RNAi沉默畸胎瘤细胞源性生长因子(PCDGF)表达对喉鳞癌Hep-2细胞增殖、凋亡及侵袭能力的影响.方法 RT-PCR检测喉鳞癌组织中PCDGF的mRNA表达水平;Hep-2细胞分为空白对照组、阴性对照组和PCDGF-siRNA组,Western blot检测转染效果及Bcl-2、Bax、E-cadherin和MUC1蛋白表达;CCK8实验检测细胞增殖;流式细胞仪检测细胞凋亡;Transwell 小室检测细胞侵袭能力.结果 喉鳞癌组织中的PCDGF mRNA表达水平显著高于声带息肉组织(t=6.88,P=0.005).PCDGF在喉鳞癌组织中高表达.PCDGF-siRNA组喉鳞癌Hep-2细胞中PCDGF、Bcl-2、MUCI蛋白表达显著低于空白对照组(P<0.05),Bax、E-cadherin蛋白表达显著高于空白对照组(P<0.05),细胞增殖率显著低于空白对照组(P<0.05),细胞侵袭数显著低与空白对照组(P<0.05),细胞凋亡率显著高于空白对照组(P<0.05).结论 抑制PCDGF的表达能够抑制喉鳞癌Hep-2细胞的增殖和侵袭能力,诱导细胞发生凋亡.

Effect of PCDGF gene silencing by RNA interference on biological characteristics of laryngeal squamous cell carcinoma Hep-2 cells

Objective To explore the effects of PCDGF on proliferation, apoptosis and invasion of laryngeal squamous cell carcinoma Hep-2 cells.Methods The mRNA expression of PCDGF in laryngeal squamous cell carcinoma was detected by RT-PCR;Hep-2 cells were divided into blank control group, negative control group and PCDGF-siRNA group;Bcl-2, Bax, E-cadherin and MUC1 protein expression were detected by Western blot;cell proliferation was detected by CCK8 assay;cell apoptosis was assessed by flow cytometry;cell invasion was determined by Transwell assay.Results PCDGF was highly expressed in laryngeal squamous cell carcinoma(t=6.88, P=0.005).PCDGF, Bcl-2 MUCI protein expression in laryngeal squamous cell carcinoma in PCDGF-siRNA group was significantly lower than that of the control group(P<0.05),Bax and E-cadherin protein expression was significantly higher than that of control group(P<0.05),cell proliferation rate was significantly lower than that of the blank control group (P<0.05),the number of cell invasion was significantly smaller than that of the blank control group(P<0.05), and the apoptosis rate was significantly higher than that of the control group (P<0.05).Conclusion Inhibiting the expression of PCDGF can block the proliferation and invasion of laryngeal squamous cell carcinoma Hep-2 cells and induce apoptosis.