游离氨基酸对牛奶蛋白过敏患儿CD4+T细胞功能影响

目的 探讨游离氨基酸配方对牛奶蛋白过敏患儿CD4+T淋巴细胞免疫功能的影响.方法 牛奶蛋白过敏患儿37例,随机分为对照组(18例)和观察组(19例).观察组患儿以游离氨基酸配方联合双歧杆菌四联活菌片治疗3个月,对照组患儿采用深度水解蛋白配方联合双歧杆菌四联活菌片治疗3个月.检测游离氨基酸配方干预前后患儿外周血淋巴细胞增殖水平、白细胞介素(IL)-17和IL-10含量水平、Th17和Treg比例.结果 治疗前淋巴细胞增殖水平、IL-17和IL-10含量以及Th17和Treg比例两组间差异无统计学意义;干预后,两组淋巴细胞增殖水平显著下降,IL-17和Th17水平显著降低,Treg和IL-10水平显著增加,且游离氨基酸配方改善CD4+T淋巴细胞免疫功能明显强于深度水解蛋白配方(P＜0.05).结论 游离氨基酸配方可显著改善牛奶蛋白过敏患儿CD4+T淋巴细胞亚群Th17和Treg之间失衡,且效果优于深度水解蛋白配方.

Effects of amino acid-based formula on CD4 + T lymphocytes function in infants with milk allergy

Objective To investigate the effect of free amino acid-based formula on CD4 + T lymphocyte immune function in infants with milk allergy.Methods The infants(37 cases) with milk protein allergy were randomly divided into control group(18 cases) and observational group (19 cases).The infants in observational group were treated with free amino acid-based formula and bifidobaeterium tetravaccine table for three months,while those in control group received extensively hydrolyzed protein formula and bifidobaeterium tetravaccine table for three months.The proliferation of peripheral blood T lymphocytes,levels of interleukin(IL)-17 and IL-10,and the ratio of Thl7 and Treg were measured in both groups before and after intervention of free amino acid-based formula recipients.Results There were no significant difference in T lymphocytes proliferation,IL-17,and IL-10 levels,and the ratio of Thl7 and Treg between two groups.After intervention,the proliferation of T lymphocytes and the levels of IL-17 and Th17 were significantly decreased,and IL-10 and Treg levels were obviously increased in both groups.Moreover,the free amino acid-based formula to improve CD4 + T lymphocyte immune function was significantly better than that of extensively hydrolyzed protein formula (P ＜ 0.05).Conclusion Amino acid-based formula can significantly improve the disequilibrium between Treg and Th17 in infants with milk allergy,and the effect is better than that of extensively hydrolyzed protein formula.