FBXO11基因对肺腺癌A549细胞迁移的影响 |
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引用本文: | 刘宁,季爽,徐曼,费广鹤. FBXO11基因对肺腺癌A549细胞迁移的影响[J]. 安徽医科大学学报, 2017, 52(4). DOI: 10.19405/j.cnki.issn1000-1492.2017.04.006 |
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作者姓名: | 刘宁 季爽 徐曼 费广鹤 |
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作者单位: | 安徽医科大学第一附属医院呼吸内科,合肥,230022;安徽医科大学第一附属医院呼吸内科,合肥,230022;安徽医科大学第一附属医院呼吸内科,合肥,230022;安徽医科大学第一附属医院呼吸内科,合肥,230022 |
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基金项目: | 国家自然科学基金,国家卫计委行业重大专项 |
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摘 要: | 目的 研究泛素连接酶复合体特异性亚基FBXO11的表达对肺腺癌A549细胞迁移的影响.方法 将A549细胞株分为3组:Blank组、阴性对照RNA组和siRNA-FBXO11组,应用Lipofectamine 2000将阴性对照RNA、siRNA-FBXO11分别转染至A549细胞中.RT-PCR法检测FBXO11的mRNA,Western blot法检测FBXO11和Snai1的蛋白表达,划痕修复实验检测细胞迁移能力.结果 与Blank组和阴性对照RNA组比较,siRNA-FBXO11组FBXO11的mRNA水平和蛋白水平显著降低(P<0.05),而Blank组与阴性对照RNA组比较,FBXO11的mRNA水平和蛋白水平无显著性差异;与Blank组和阴性对照RNA组比较,siRNA-FBXO11组的Snail蛋白表达水平显著增加(P<0.05),而Blank组与阴性对照RNA组比较,Snail蛋白表达水平无显著性差异;划痕实验结果显示:与Blank和阴性对照RNA组比较,siRNA-FBXO11组的细胞迁移距离显著增加(P<0.05),而Blank组与阴性对照RNA组比较,细胞迁移距离无显著性差异.结论 FBXO11抑制肺腺癌A549细胞的迁移能力,这可能与Snail调控有关.
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关 键 词: | 肺腺癌 FBXO11 Snail 迁移 |
Effect of FBXO11 gene on migration of lung adenocarcinoma A549 cells |
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Abstract: | Objective To explore the effects of the ubiquitin ligase complex specificity subunit FBXO11 on lung adenocarcinoma A549 cells migration.Methods The A549 cell line was cultured and divided into blank group, negative control RNA group and siRNA-FBXO11 group.Negative control RNA and siRNA-FBXO11 were transfecected into A549 cells by Lipofectamine 2000 respectively.Levels of mRNA of FBXO11 was measured by RT-PCR and the expression of FBXO11 and Snail was detected by Western blot in the transfected cells.The migration of A549 cells was evaluated by wound healing assay.Results Compared with blank group and negative control RNA group, the levels of mRNA and the expression of FBXO11 in siRNA-FBXO11 group was down-regulated significantly(P<0.05),and compared with blank group and negative control RNA group,there was no statistically significant difference.Compared with blank group and negative control RNA group,the expression of Snail in siRNA-FBXO11 group was increased markerdly(P<0.05), and compared with blank group and negative control RNA group, there was no statistically significant difference.Compared with blank group and negative control RNA group, the migration distance in siRNA-FBXO11 group was increased significantly(P<0.05), and compared with blank group and negative control RNA group, there was no statistically significant difference.Conclusion FBXO11 might inhibit the migration of lung adenocarcinoma A549 cells via the regulation of Snail. |
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Keywords: | lung adenocarcinoma FBXO11 Snail migration |
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