| 高效液相色谱法测定异叶爬山虎中异槲皮苷的含量 |
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| 引用本文： | 张颖,李嘉,何春欢,陈锋,姜平川.高效液相色谱法测定异叶爬山虎中异槲皮苷的含量[J].安徽医药,2017,21(1):46-48. |
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| 作者姓名： | 张颖 李嘉 何春欢 陈锋 姜平川 |
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| 作者单位： | 广西壮族自治区中医药研究院化学所,广西南宁 530022;广西中药质量标准研究重点实验室,广西南宁 530022;广西壮族自治区中医药研究院化学所,广西南宁 530022;广西中药质量标准研究重点实验室,广西南宁 530022;广西壮族自治区中医药研究院化学所,广西南宁 530022;广西中药质量标准研究重点实验室,广西南宁 530022;广西壮族自治区中医药研究院化学所,广西南宁 530022;广西中药质量标准研究重点实验室,广西南宁 530022;广西壮族自治区中医药研究院化学所,广西南宁 530022;广西中药质量标准研究重点实验室,广西南宁 530022 |
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| 基金项目： | 广西壮族自治区科技厅项目，广西中药质量标准研究重点实验室课题 |
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| 摘 要： | 目的 建立高效液相色谱法(HPLC)测定异叶爬山虎中异槲皮苷含量的方法,并测定广西不同地区的异叶爬山虎中异槲皮苷的含量.方法 采用Inersil-C18色谱柱(4.6 mm×25 mm,5μm),流动相为甲醇-0.05%磷酸溶液(46:54),流速1 mL·min-1,检测波长258 nm,柱温25℃,进样量10μL,分析时间32 min.结果 异槲皮苷的进样量在20~200μg范围内,与峰面积呈良好的线性关系,相关系数r=0.9999(n=6);异槲皮苷的平均回收率为98.53%(n=6),RSD为0.79%.结论 该法操作简便,重复性好,可用于测定异叶爬山虎中异槲皮苷含量.
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| 关 键 词： | 异叶爬山虎 异槲皮苷 高效液相色谱法 含量测定 |
| 收稿时间： | 2016/8/31 0:00:00 |
| 修稿时间： | 2016/10/21 0:00:00 |
Determination of the content of isoquercitrin in Parthenocissus heterophylla (BI.) by HPLC |
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| ZHANG Ying,LI Ji,HE Chunhuan,CHEN Feng and JIANG Pingchuan.Determination of the content of isoquercitrin in Parthenocissus heterophylla (BI.) by HPLC[J].Anhui Medical and Pharmaceutical Journal,2017,21(1):46-48. |
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| Authors: | ZHANG Ying LI Ji HE Chunhuan CHEN Feng and JIANG Pingchuan |
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| Institution: | Department of Chemistry,Guangxi Institute of Chinese Medicine Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China,Department of Chemistry,Guangxi Institute of Chinese Medicine Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China,Department of Chemistry,Guangxi Institute of Chinese Medicine Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China,Department of Chemistry,Guangxi Institute of Chinese Medicine Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China and Department of Chemistry,Guangxi Institute of Chinese Medicine Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China |
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| Abstract: | Objective To establish a method for the determination of Isoquercitrin in EAE extract of Parthenocissus heterophylla (BI.)Merr.Methods The Component was separated on a Inersil C18 column (4.6 mm ×25 mm,5 μm)with a flow-rate of 1 mL·min -1 at25 ℃.The mobile phase consisted a mixture of methanol and 0.05% phos phoric acid (46:54).The total analysis time was 32 min.The detector was DAD and the detection wavelength was 258 nm.Results The linear range of Isoquercitrin was 20 ~200 μg,(r =0.9999).The average recovery was 98.53%,RSD was 0.79% (n =6).Conclusion The method is simple and accurate,and can beused for the determination of content of Isoquercitrin in the EAE extract of Parthenocissus heterophylla (BI.)Merr. |
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| Keywords: | Parthenocissus heterophylla Isoquercitrin HPLC Content determination |
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