miRNA-143对人鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响

目的 探讨miRNA-143对鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响.方法 利用荧光定量PCR检测人永生化鼻咽上皮细胞(NP69)、高分化鼻咽癌细胞(CNE-1)、低分化鼻咽癌细胞(CNE-2Z)中miRNA-143的表达水平.利用感染慢病毒的方式建立稳定过表达miRNA-143的CNE-2Z细胞,并用荧光定量PCR法进行检测.通过CCK-8法检测过表达miRNA-143对CNE-2Z细胞增殖的影响,通过Transwell迁移和侵袭试验分析过表达miRNA-143对CNE-2Z细胞迁移和侵袭的影响.结果 低分化鼻咽癌细胞(CNE-2Z)中miRNA-143的表达水平显著低于CNE-1和NP69细胞(P<0.01).稳定过表达miRNA-143的CNE-2Z细胞(CNE-2Z/miR-143)中miRNA-143的表达水平显著高于CNE-2Z细胞(P<0.01)和慢病毒对照组细胞(CNE-2Z/miR-NC)(P<0.01).细胞增殖能力检测显示,与CNE-2Z组和CNE-2Z/miR-NC组相比,CNE-2Z/miR-143组在72 h和96 h时细胞活力显著降低(P<0.05,P<0.01).Transwell迁移和侵袭试验结果显示,CNE-2Z/miR-143组与CNE-2Z组和CNE-2Z/miR-NC组相比细胞迁移和侵袭能力显著下降(P<0.01).结论 miR-143能够抑制鼻咽癌细胞CNE-2Z的增殖、迁移和侵袭能力,提示其可能对鼻咽癌的诊断、治疗及预后评价有重要意义.

Effect of miRNA-143 on proliferation,migration and invasion of CNE-2Z cells of human nasopharyngeal cancer

Objective To detect the effects of miR-143 on proliferation, migration and invasion of human nasopharyngeal cancer CNE-2Z cells.Methods The expression of miR-143 in NP69 cells, CNE-1 cells and CNE-2Z cells were detected by using real-time PCR.The overexpression of miR-143 in CNE-2Z cells was constructed through infecting lentivrius, and the level of miR-143 was determined by using real-time PCR.Cell viability was detected by using a CCK-8 kit according to the manufacturer's instruction at indicated time points.The effectiveness of overex-pression of miR-143 on migration and invasion of CNE-2Z cells were detected by using Transwell cell migration and invasion assay.Results The expression level of miR-143 in CNE-2Z was significantly lower than those in NP69 and CNE-1 (P<0.01).The miR-143 over-expressed CNE-2Z cell was successfully established.The cell viability in CNE-2Z/ miR-143 group was significantly decreased compared with CNE-2Z and CNE-2Z/miR-NC (P<0.01).Overexpression of miR-143 inhibited cell migration and invasion of CNE-2Z cells significantly(P<0.01).Conclusion miR-143 might inhibit cell proliferation, migration and invasion in human nasopharyngeal cancer CNE-2Z cells, indicating its important role in diagnosis, treatment and prognosis of nasopharyngeal cancer.