| 高效液相色谱法测定消炎解毒软膏中华蟾酥毒基和脂蟾毒配基的含量 |
| |
| 引用本文： | 陈士翠,鞠建明.高效液相色谱法测定消炎解毒软膏中华蟾酥毒基和脂蟾毒配基的含量[J].中南药学,2008,6(2):176-178. |
| |
| 作者姓名： | 陈士翠 鞠建明 |
| |
| 作者单位： | 金湖食品药品监督管理局,江苏金湖,211600 |
| |
| 摘 要： | 目的建立消炎解毒软膏中华蟾酥毒基和脂蟾毒配基含量测定方法。方法采用HPLC法测定消炎解毒软膏中华蟾酥毒基和脂蟾毒配基的含量,色谱条件为Alltima C18：色谱柱（4.6mm×250mm,5μm）;流动相：水-乙腈（50∶50）,流速：1mL·min-1;检测波长：296nm;柱温：35℃。结果华蟾酥毒基的进样量在0.427～17.080μg峰面积积分值与进样量有良好的线性关系（r=0.9999）,平均回收率为98.3%,RSD为2.0%（n=6）;脂蟾毒配基的进样量在0.262～10.480μg峰面积积分值与进样量有良好的线性关系（r=0.9999）;平均回收率为98.9%,RSD为1.8%（n=6）。结论本方法简便、快速准确、灵敏度高、重现性好,可作为该制剂的含量测定方法。
|
| 关 键 词： | 消炎解毒软膏 华蟾酥毒基 脂蟾毒配基 高效液相色谱法 |
| 文章编号： | 1672-2981(2008)02-0176-03 |
| 修稿时间： | 2008年1月26日 |
Content determination of cinobufagin and resibufogenin in Xiaoyan Jiedu ointment by HPLC |
| |
| CHEN Shi-cui,JU Jian-ming.Content determination of cinobufagin and resibufogenin in Xiaoyan Jiedu ointment by HPLC[J].Central South Pharmacy,2008,6(2):176-178. |
| |
| Authors: | CHEN Shi-cui JU Jian-ming |
| |
| Institution: | CHEN Shi-cui , JU Jian- ming(Food and Durg Administration of Jinhu,Jiangsu Province Jinhu.Jinhu Jiangsu 21600;Jiangsu Acadeny of Traditionat Chinese Medicine ,Nanjing 210028) |
| |
| Abstract: | Objective To establish a method for the content determination of cinobufagin and resibufogenin in Xiaoyan Jiedu ointment by HPI,C. Methods Chromatography was performed on an Alltima C18 colunm （4.6 min×250 mm, 5 μm）. The mobile phase was water-acetonitrile （50 ： 50, v/v）, the flow rate was 1 mL · min^- 1 , the detective wavelength was 296 nm, and the colum temperature was 35 C. Results The linear ranges of cinobufagin and resibufogenin were 0. 427-17. 080μg （r=0.9,99 9） and 0. 262-10. 180μg （r=0. 999 9）. The average reeovery of cinobufagin and resibufogenin was 98.3% （RSD 2.0%） and 98.9% （RSD= 1.8%. n= 6）. Conclusion The method is simple,accurate, sensitive, reliable and can be used for the quality control of Xiaoyan Jiedu ointment. |
| |
| Keywords: | Xiaoyan Jiedu Ointment cinobufagin resibufogenin HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
