前列腺癌中白细胞介素-6的表达及其意义

目的 探讨细胞因子白细胞介素-6(IL-6)在前列腺癌发生发展中的作用及其临床意义.方法 ①采用免疫组化SABC法和逆转录-聚合酶链反应(RT-PCR)法对前列腺癌组织及其相应的癌旁前列腺组织和前列腺癌细胞系PC-3及LNCaP中的IL-6表达进行检测;②采用酶联免疫吸附试验(ELlSA)检测前列腺癌患者及随机正常人群外周血中IL-6的浓度和前列腺癌细胞系PC-3及LNCaP培养上清液中的IL-6浓度.结果 ①前列腺癌组织中IL-6表达明显强于相应的癌旁前列腺组织,且与肿瘤的分期分级相关;PC-3细胞中IL-6呈强阳性表达,而LNCaP细胞中IL-6呈弱阳性表达.②前列腺癌患者外周血中IL-6浓度显著高于正常人群组;而PC-3细胞组培养上清液中IL-6浓度也明显高于LNCaP细胞组.结论 IL-6基因可能在前列腺癌的发生发展中起重要作用,有可能是前列腺癌从激素依赖性转化为非激素依赖性的因素之一.

The Expression and Significance of Interleukin-6 in Prostate Cancer

Objective To study the role of interleukin-6(IL-6)in the pathogenesis of prostate cancer and its clinical significance. Methods Immunohistochemistry and RT-PCR were used to detect the expression of IL-6 protein and mRNA in frozen prostatic adenocarcinoma,adjacent benign prostatic tissue,and prostate cancer cell lines PC-3 and LNCaP. The serum levels of IL-6 in patients with prostate cancer and healthy controls,and the supernatants of prostate cancer cell cultures were measured by using ELISA. Results The IL-6 protein levels in prostate cancer tissue and PC-3 cells were significantly higher than those in adjacent benign prostatic tissue and LNCaP cells. The serum IL-6 levels in the patients with prostate cancer were markedly higher than those in the healthy controls. The IL-6 levels in supernatants in PC-3 cells were notably higher than those in the LNCaP cells. Conclusion The IL-6 gene may act as an important regulator in prostate cancer progression and may be one of the causes of prostate cancer conversion from an initially androgen-dependent state into an androgen-independent state.