大黄HPLC指纹图谱分析

 目的采用高效液相色谱法建立大黄药材的指纹图谱,为其质量控制提供依据。方法Kromasil C₈(4.6mm×200mm,5μm)色谱柱,甲醇-0.4%冰醋酸溶液梯度洗脱,流速0.8mL·min^-1,检测波长254nm。结果通过聚类分析,14个正品大黄样品被聚为2类,非正品被分为4类。通过相似度计算,14个正品大黄样品的相似度均在0.7以上,其余非正品的相似度均小于0.60。结论本方法可用于大黄的真伪鉴别和质量评价。

Fingerprints analysis of Radix et Rhizoma Rhei by HPLC

OBJECTIVE To develop the fingerprint of Radix et Rhizoma Rhei by high-performance liquid chromatography.METHODS The Kromasil C₈(4.6 mm×200 mm ,5 μm) column was used with a mobile phase of methanol and 0.4% acetic acid aqueous in gradient mode. The flow rate was 0.8 mL·min^-1, and the detection was set at 254 nm.RESULTS By using cluster analysis and similarity calculation, the fourteen samples from Chinese pharmacopoiea recorded species were classified as two clusters with similarities above 0.7 while others were classified as four different clusters with similarities less than 0.60. CONCLUSION This method can be used for identification and quality evaluation of Radix et Rhizoma Rhei.