青海地区汉、藏族PLCE1rs2274223基因多态性与胃癌相关性

目的 通过检测PLCE1 rs2274223基因在青海汉、藏族胃癌患者与健康对照者中的分布,初步探讨其多态性与汉、藏族胃癌的相关性.方法 提取青海汉、藏族胃癌患者与健康人群的外周血DNA,采用dHPLC法行PLCE1 rs2274223基因位点分型.并行相关分析.结果 汉族胃癌组PLCE1基因单核苷酸多态性位点rs2274223的基因型（AA,AG,GG）频率分别为53.33%、42.50%、4.17%,对照组为56.67%、38.33%、5.00%,两组间各基因型分布差异无统计学意义（P＞0.05）.藏族胃癌组PLCE1 rs2274223基因的基因型（AA,AG,GG）频率分别为50.00%、40.83%、9.17%,对照组为60.83%、36.67%、2.50%,两组间AA、AG基因型分布差异无统计学意义（P＞0.05）,两组间GG基因型分布差异有统计学意义（P＜0.05）,与AA、AG型比较,携带GG基因型者胃癌发生的危险性增加（OR=3.936,95%CI=1.069-14.485）.两民族间PLCE1 rs2274223的3种基因型（AA,AG,GG）频率差异无显著性（P＞0.05）.结论 本研究显示,两民族间PLCE1基因rs2274223的基因型（AA,AG,GG）分布频率不存在差异.PLCE1基因 rs2274223位点单核苷酸多态性与青海地区汉族人群胃癌易感性不存在相关性.携带GG基因型的青海地区藏族人群胃癌发病风险较高.

A STUDY ON THE RELATIONSHIP BETWEEN PLCE1 rs2274223 GENE POLYMORPHISMS AND SUSCEPTIBILITY TO GASTRIC CANCER IN HAN AND TIBETAN NATIONALITIES IN QINGHAI AREA

Objective To investigate the polymorphisms of PLCE1 gene rs2274223 and the relationship between the PLCE1 rs2274223 genotype and susceptibility to gastric cancer in Han and Tibetan nationalities in Qinghai area.Methods Genotypes were determined by PCR-based denaturing high-performance liquid chromatography analysis and direct DNA sequencing in 120 cases with gastric cancer and 120 controls recruited in Han and Tibetan nationalities,respectively.Results In patients and controls in Han nationality,the frequency of AA was 53.33% and 56.67%,respectively,AG was 42.50%and 38.33%,respectively,GG was 4.17% and 5.00%,respectively.NO significant difference was found in the genotypes frequency between the gastric cancer group and the control group（P〉0.05）.In patients and controls of Tibetan nationality,the frequency of AA was 50.00% and 60.83%,respectively,AG was 40.83% and 36.67%,respectively,GG was 9.17% and 2.50%,respectively.NO significant difference was found in the AA/AG genotypes frequency between the gastric cancer group and the control group（P〉0.05）,but there was a significant difference between patients and control in GG genotype（P〈0.05）.Genotypes of Han and Tibetan nationalities showed no difference.Conclusion The polymorphism of PLCE1 gene rs2274223 GG genotype are associated with the susceptibility of Tibetan gastric cancer but not with Han gastric cancer in Qinghai area, PLCE1 rs2274223 genotypes of Han and Tibetan nationalities showed no difference.