急进高原大鼠形态学变化与早期脑水肿关系的研究

目的 为阐明急进高原脑组织病变发病机制,对急进高原大鼠在高原环境下脑组织形态学变化和脑含水量的改变进行相关研究.方法 解剖、观察Wister大鼠高原1、3 、7 d组脑组织大体形态变化.H-E染色,光镜观察脑组织学变化;电镜观察脑组织超微结构变化.测定脑组织含水量.结果 光镜下,脑组织出现明显炎症反应,脑组织充血水肿,内有大量的炎性细胞.电镜下,神经元细胞出现凋亡.脑组织含水量与对照组比较,1、3、7 d组含水量增加（P＜0.05）.结论 高原脑组织损伤的发生与高原缺氧有关.推测缺氧使脑毛细血管壁通透性增加、脑水转运功能障碍;脑细胞发生凋亡,脑组织内液体大量聚集,引发脑水肿.

THE RELATIONSHIP BETWEEN MORPHOLOGICAL CHANGES AND EARLIER CEREBRAL EDEMA IN RATS ACCELERATED AT HIGH ALTITUDE

Objective To investigate the relationship between morphological changes and earlier cerebral edema in rats accelerated at high altitude.Methods To divide the Wister rats into 3 groups（1d,3d and 7d）at plateau randomly and to observe the morphological changes of cerebral tissue by H-E staining.The brain histological changes were observed by microscope,the morphological changes of brain tissue were observed by Electron microscope.And at the same time,water contents of brain was determined.Results There were significant inflammatory reaction in cerebral tissue.Congestion and edema were shown in brain tissue and a great amount of inflammatory cells presented brain space under microscope.The apoptosis cells were shown under electron microscope.Cerebral water contents were increased in 1d,3d and 7d groups compared with control group（P〈0.05）.Conclusions High altitude may cause cerebral tissue damage because of hypoxia,suggesting that hypoxia may increase brain capillary wall permeability and dysfunction of brain water transportation.