胶原涂层煅烧骨-成骨细胞复合物的体内成骨作用

目的观察胶原涂层煅烧骨-成骨细胞复合物植入体内后成骨性能,探讨治疗骨缺损的新方法。方法以牛松质骨制成煅烧骨(TBC),并涂以胶原制成胶原涂层煅烧骨(TBCc),培养兔骨膜成骨细胞(POB),构建复合物TBCc-POB;将该复合物移植到BALB/c裸小鼠背部右侧皮下,4周取材;同时将该复合物植入兔背部右侧肌袋内,8周取材。两种动物均在对侧相应部位植入单纯TBCc作为对照,并在相应时间内取组织块常规处理后光镜、电镜观察。结果植入材料在皮下及肌袋处均有成骨细胞大量生长、增殖,并有软骨组织、骨组织形成,而对照组则没有。结论将TBCc-POB 材料植入体内后,成骨细胞能够存活并产生软骨组织和骨组织。

In vivo osteogenic properties of the composite of periosteal-derived osteoblast and collagen coated true bone ceramics

Objective To contrive new ways for repairing bone defects on the basis of the observation of the osteogenic properties of implanted composite made from periosteal-derived osteoblasts (POB) and true bone ceramics (TBC) coated with collagen (TBCc). Methods Bovine cancellous bone was calcined and coated with collagen before being mixed with cultured rabbit osteoblast suspension to prepare the composite, which was respectively implanted subcutaneously on the right of the back of BALB/c nude mice and in the muscle pouches in similar sites of rabbits. The control was established by implantation of TBCc in the contralateral sites corresponding to the former implants in both of animals. Samples were obtained from the implant sites 4 weeks later in mice and 8 weeks later in rabbits respectively, and light and electron microscopic observation was performed routinely. Results A large number of osteoblasts were seen to grow and proliferate within the TBCc-POB composite where cartilage and bone tissues also generated, which was no observed in the control group. Conclusion The osteoblasts in the implanted TBCc-POB composite can survive and develop into cartilage and bone tissues.