| 人参蛋白对Hep-细胞增殖影响的比较 |
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| 引用本文： | 李红艳,赵雨,张惠,幺宝金,王伟楠. 人参蛋白对Hep-细胞增殖影响的比较[J]. 中国民族民间医药杂志, 2010, 19(1): 33-34 |
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| 作者姓名： | 李红艳 赵雨 张惠 幺宝金 王伟楠 |
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| 作者单位： | [1]长春中医药大学研发中心,吉林长春130117 [2]吉林大学公共卫生学院,吉林长春130021 |
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| 基金项目： | 国家自然科学基金项目(30873371);;国家科技支撑计划(2007BAI38B02) |
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| 摘 要： | 目的：比较人参蛋白对人喉癌细胞（Hep-2）增殖的影响。方法：采用中性缓冲液浸提法制备人参蛋白I，蛋白I经沸水浴处理、等电点沉淀，分别制备得到蛋白Ⅱ、Ⅲ、IV；采用MTT法检测细胞活性。结果：人参蛋白I及人参蛋白Ⅲ对Hep-2细胞增殖具有明显抑制作用（P〈0．01），人参蛋白Ⅱ及人参蛋白Ⅳ对Hep-2细胞增殖无明显影响。结论：人参蛋白是人参的主要活性成分之一，其对Hep-2细胞增殖的影响与蛋白制备方法密切相关。
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| 关 键 词： | 人参蛋白 Hep-2 增殖 比较 |
Compare the efect of ginseng protein on the proliferation of human laryngeal carcinoma Hep-2 cells |
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| LI Hong-yan,ZHAO Yu,ZHANG Hui,YAO Bao-jin,Wang Wei-nan.Center for New Medicine Research of Changchun University of Traditional Medicine,Changchun ,China,.School of Public Health,Jilin University,Changchun. Compare the efect of ginseng protein on the proliferation of human laryngeal carcinoma Hep-2 cells[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2010, 19(1): 33-34 |
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| Authors: | LI Hong-yan ZHAO Yu ZHANG Hui YAO Bao-jin Wang Wei-nan.Center for New Medicine Research of Changchun University of Traditional Medicine Changchun China .School of Public Health Jilin University Changchun |
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| Affiliation: | LI Hong-yan1,ZHAO Yu1,ZHANG Hui1,YAO Bao-jin2,Wang Wei-nan11.Center for New Medicine Research of Changchun University of Traditional Medicine,Changchun 130117,China,2.School of Public Health,Jilin University,Changchun,130021 |
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| Abstract: | Objective To compare the elect of ginseng protein on the proliferation of human laryngeal carcinoma Hep - 2 cells. Methods Using the methods of saltsolution to extract the protein I from ginseng, then using the methods of boiling water bath and Isoelectrie precipitation to extract the protein Ⅱ, Ⅲ and IVfrom protein I .The effect of the ginseng protein on the proliferation of Hep - 2 cells was determined with the method of Mqq＇. Results The protein I and protein Ⅲ could inhibit the proliferation of Hep -2 cells （P 〈0. 01 ）, but the proliferation of Hep- 2 cells were affected inconspicuously by protein Ⅱ and protein IV. Conclusion Ginseng protein is the main active component of ginseng, and the elect of ginseng protein on the proliferation of Hep - 2 cells closely related to the extract method of protein. |
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| Keywords: | ginseng protein Hep-2 proliferation compare |
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