去细胞结合光氧化技术处理牛颈静脉抗钙化性能研究

目的 探讨去细胞结合光氧化技术处理后的牛颈静脉带瓣管道(BJVC)在体内的抗钙化性能.方法 取新鲜牛颈静脉20根,每根裁出4个带瓣血管片,随机分为A、B、c、D 4组,分别以染料介导光氧化、戊二醛、去细胞及去细胞结合光氧化4种技术处理.建立大鼠皮下包埋模型,2个月后获取组织标本,原子吸收光谱法测定组织钙含量;并应用X线、CT、骨密度扫描检测整体组织钙化情况.结果 (1)D组试片标本形态较完整,柔韧性好,无结节样改变;其整体组织钙盐密度明显低于A组及B组,与C组接近.(2)D组管壁组织钙含量显著低于A组及B组(P＜0.01),与C组差异无统计学意义(P＞0.05);D组瓣膜组织的钙含量显著低于B组(P＜0.01),与A组及c组比较均差异无统计学意义(P＞0.05).结论 去细胞结合光氧化技术与传统戊二醛及染料介导光氧化技术比较,能明显提高牛颈静脉体内抗钙化性能.

The anti-calcification properties of BJVC treated with dye-mediated photooxidation following decellularization

Objective To study the anti-calcification properties of BJVC treated by dye-mediated photooxidation following decellularization in vivo.Methods Each of twenty fresh bovine jugular veins with a retained native valve procured from a slaughterhouse was cut into 4 trial patches with valve,which were randomly divided into greup A,B,C and D,which were treated respectively by the technology of dye-mediated photooxidation,glutaraldehyde,decellularization and dye-mediated photooxidantion following decellularization.One of each group trial patches were implanted subcutaneously in the same Wistar rat.60 days after implantation,all trial rats were sacrificed and the specimens were retrieved.The tissue calcium content was determined by flame atomic absorption spectrophotometer.The tissue calcium salt distribution was evaluated by X-ray,spiral computed tomography,bone density scanning.Results (1)The trial patches in D group were relative integrity,soft and tenacious,no nodes emerged,its calcium salts density in total was significantly less than those of group A and B,and was similar to group C. (2)The calcium content of vessel wall in group D was significantly lower than those of group A and B (P＜0.01),and was no different with which of group (P＞0.05).The calcium content in the valves in group D was lower than that of group B(P＜0.01),and was no different with those of group A and D (P＞O.05).Conclusion The technology of dye-mediated photooxidation following decellarization can effectively prevent the calcification of bovine jugular venous conduit in vivo.