| 血细胞的Percoll不连续密度梯度分离法 |
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| 引用本文： | 顾庆礼,张菁,张健.血细胞的Percoll不连续密度梯度分离法[J].苏州大学学报(自然科学版),1991(1). |
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| 作者姓名： | 顾庆礼 张菁 张健 |
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| 作者单位： | 江苏省血液研究所
(顾庆礼，张菁)，江苏省血液研究所(张健) |
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| 摘 要： | 以Percoll作为分离介质,采用60%SIP、50%SIP2种浓度,经2次离心,成功地分离了12名健康供血者周周血的单核、淋巴和粒细胞,纯度分别为83%、85%和98%;得率分别为48%、49%和47%。常规涂片、瑞氏染色后形态学观察示细胞形态完整,台盼蓝拒染实验示细胞活力良好(拒染率>95%).
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| 关 键 词： | Percoll 不连续密度梯度离心 聚蔗糖—泛影葡胺 血细胞分离 |
Discontinuous Density Gradient Centrifugal Method for the Human Blood Cell Separation Using Percoll Medium |
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| Gu Qingli,et al.Discontinuous Density Gradient Centrifugal Method for the Human Blood Cell Separation Using Percoll Medium[J].Suzhou University Journal of Medical Science,1991(1). |
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| Authors: | Gu Qingli |
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| Institution: | Jiangsu Institute of Hematology |
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| Abstract: | The authors report a discontinuous density gradient centrifugation technique for separatingthe human peripherial white blood cells into monocytes,lymphocytes,and granulocytes by meansof percoll as a purifying medium.The consecutive centrifugation was performed in SIP at a concentration of 60% and 50% for 2 times.The data indicate that the purity and recovery rate are83%,85% and 98%,and 48%,49% and 47% respectively.The cell smear shows that cellsare intact(Wright staining).The Trypane Blue exclusion test demonstrates a good cell viabilitywith an exclusion rate of more than 95%. |
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| Keywords: | percoll ficoll-hypaque discontinuous density gradient centrifugation blood cell separation |
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