茵陈蒿的化学成分及其药理活性研究

目的 研究传统中药材茵陈蒿的化学成分，以期从中发现具有药理活性的先导化合物。方法 利用大孔吸附树脂、硅胶、ODS等柱色谱与半制备型高效液相色谱相结合对茵陈蒿中的化合物进行分离纯化，并结合化合物的理化性质与核磁共振波谱数据对分离得到的化合物进行结构鉴定。此外，采用MTT法和ELISA法对分离得到的化合物进行了抗肝癌以及抗HBV活性测试。结果 从采自山西的茵陈蒿的乙醇提取物中分离出10个单体化合物，活性测试结果表明上述化合物未显示抗肝癌以及抗HBV活性。结论 化合物1和2为新化合物，化合物1,3,4为烯炔类化合物，化合物2和10为脂肪酸类化合物，化合物5-9为萜类化合物。

Study on Chemical Constituents and Pharmacological Activities of Artemisia Capillaris

Objective To investigate the chemical constituents of Artemisia capillaris, traditional Chinese herb, with a view to discovering the lead compounds with pharmacological activities. Methods The chemical constituents were isolated and purified by the combination of macroporous adsorption resin, silica gel, ODS column chromatography and semi-preparative high performance liquid chromatography. The structures of the isolates were identified based on the physico-chemical properties and NMR spectroscopy data. In addition, the anti-hepatocellular carcinoma and anti-HBV activities of the isolated compounds were tested by MTT assay and ELISA. Results Ten compounds were isolated from the ethanol extract of Artemisia capillaris. Bioassay screening indicated that those isolates were not active. Conclusion Compounds 1 and 2 were new compounds, compounds 1, 3, and 4 are polyacetylenes, compounds 2 and 10 are aliphatic acid, and compounds 5-9 are terpenoids.