Orexin A associates with inflammation by interacting with OX1R/OX2R receptor and activating prepro-Orexin in cancer tissues of gastric cancer patients |
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| Institution: | 1. Digestive division, Endoscopic center, People''s Hospital of Ningxia Hui Autunomous Region, Yinchuan, China;2. Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, China;3. 521 Hospital of Norinco Group, Xi’an, China;1. Servicio de Aparato Digestivo, Hospital Galdakao-Usansolo, Galdakao, España;2. Biocruces Bizkaia Health Research Institute, Barakaldo, España;3. Laboratorio de Microbiología, Hospital Galdakao-Usansolo, Galdakao, España;4. Unidad de Investigación. Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), España;1. Unidad de Endoscopia Digestiva, Hospital Universitari i Politècnic La Fe, Grupo de Investigación de Endoscopia Digestiva, IIS La Fe, Valencia, España;2. Centro de Visión por Computador, Departamento de Ciencias de la Computación, Universidad Autónoma de Barcelona, Barcelona, España;3. Unidad de Endoscopia, Servicio de Gastroenterología, Hospital Clínic, IDIBAPS, CIBEREHD, Universidad de Barcelona, Barcelona, España;4. IDIBAPS, CIBEREHD, Barcelona, España;1. Department of Internal Medicine, Division of Gastroenterology, Van Education and Research Hospital, Van, Turkey;2. Department of Pediatry, Van Education and Research Hospital, Van, Turkey;3. Department of Psychiatry, BezmialemVakıf University, Istanbul, Turkey;1. Department of Digestive Diseases, Miguel Servet University Hospital, Zaragoza, Spain;2. Aragón Health Research Institute (IIS Aragón), Spain;1. Servicio de Cirugía General y del Aparato Digestivo, Hospital Universitario de Canarias, La Laguna, Santa Cruz de Tenerife, España;2. Servicio de Radiología Intervencionista, Hospital Universitario de Canarias, La Laguna, Santa Cruz de Tenerife, España;1. Chongqing Medical University, China;2. Chongqing Three Gorges University, China |
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| Abstract: | ObjectiveGastric cancer (GC) has been become the second leading cause for cancer-associated death. This study aimed to investigate Orexin A levels and associated receptors in tumor tissues of GC patients.Patients and methodsForty-six consecutive gastric cancer patients (GC, n = 46) and 13 chronic atrophic gastritis patients (CAG, n = 13) were recruited. Meanwhile, 18 health individuals visiting Medical Examination Department were involved as control (N group, n = 18). ELISA was used to examine Orexin A concentration. Immunohistochemistry assay was used to examine OX1R and OX2R. HE staining was applied to evaluate inflammation. qRT-PCR was employed to detect OX1R, OX2R, prepro-Orexin mRNAs. Serum Helicobacter pylori (H. pylori) infection was measured.ResultsOrexin A expression in GC patients was significantly up-regulated compared to N group and CAG group (p < 0.05). Orexin A expression was increased in CAG group compared to N group (p < 0.05). Gastric cancer tissues exhibited significantly obvious inflammation compared to N group and CAG group (p < 0.05). OX1R and OX2R expressions were significantly down-regulated in GC group compared to N group and CAG group (p < 0.05). OX1R and OX2R were lower significantly in GC group compared to CAG group (p < 0.05). Prepro-Orexin was significantly depleted in tumor tissues of GC group compared to N group and CAG group (p < 0.05). Orexin A expression was un-associated with gender, age and differential grades (p > 0.05). CAG and GC patients demonstrated higher H. pylori infection rates.ConclusionOrexin A was associated with inflammation by interacting with OX1R/OX2R receptor and activating prepro-Orexin in tumor tissues of gastric cancer patients. |
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| Keywords: | Orexin A OX1R Prepro-Orexin Gastric cancer Orexina-A OX1R Prepo-orexina Cáncer gástrico |
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