| 胆红素对神经突触膜Na^＋,K^＋—ATP酶影响的实验研究 |
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| 引用本文： | 邵洁 俞善昌. 胆红素对神经突触膜Na^＋,K^＋—ATP酶影响的实验研究[J]. 中华儿科杂志, 1997, 35(10): 524-526 |
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| 作者姓名： | 邵洁 俞善昌 |
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| 作者单位： | 上海第二医科大学附属瑞金医院儿内科 |
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| 摘 要： | 为了研究胆红素的神经毒性作用，对５９只出生２天的ＳＤ大白鼠分别予以腹腔注射不同剂量胆红素制造高胆红素血症动物模型，不连续密度梯度法分离提取脑神经元突触膜，测定Ｎａ＋，Ｋ＋－ＡＴＰ酶活力。结果：随着血清胆红素浓度的逐渐增加，脑组织中胆红素沉积量也逐渐增加，神经元突触膜Ｎａ＋，Ｋ＋－ＡＴＰ酶活力逐渐降低，Ｎａ＋，Ｋ＋－ＡＴＰ酶活力与脑组织中沉积的胆红素量呈负相关（ｒ＝－０．３４，Ｐ＜０．０１）。提示：胆红素对Ｎａ＋，Ｋ＋－ＡＴＰ酶具有抑制作用。
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| 关 键 词： | 高胆红素血症 突触膜 NA（＋）K（＋）交换ATP酶 |
Effect of bilirubin on Na ,K ATPase of synaptic membrane |
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| Shao Jie,Yu Shanchang,Li Yunzhu,et al.. Effect of bilirubin on Na ,K ATPase of synaptic membrane[J]. Chinese journal of pediatrics, 1997, 35(10): 524-526 |
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| Authors: | Shao Jie Yu Shanchang Li Yunzhu et al. |
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| Affiliation: | Shao Jie,Yu Shanchang,Li Yunzhu,et al. Department of Pediatrics,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025 |
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| Abstract: | The aim of this study was to understand the neurotoxicity of bilirubin. In this study 2 day old Sprague Dawley rats adminstrated with bilirubin were recruited as the model of neonatal hyperbilirubinemia. Synaptic membrane Na ,K ATPase activities were determined in brain homogenates of rats by using direct spectrophotometry, chloroform extraction method and discontinuous density gradient centrifugation. The results showed that with the increase in serum bilirubin levels, the amount of brain bilirubin was gradually increased, but synaptic membrane Na ,K ATPase activities were gradually decreased. There was a negative correlation between the amount of bilirubin deposition in the brain and Na ,K ATPase activities. |
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| Keywords: | Hyperbilirubinemia Synaptic membranes Na( ) K( ) exchanging ATPase |
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