Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain |
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Authors: | Martella V Ciarlet M Bányai K Lorusso E Cavalli A Corrente M Elia G Arista S Camero M Desario C Decaro N Lavazza A Buonavoglia C |
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Institution: | Department of Animal Health and Well-being, University of Bari, Valenzano, Bari, Italy. v.martella@veterinaria.uniba.it |
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Abstract: | Rotavirus genome segment 4, encoding the spike outer capsid VP4 protein, of a porcine rotavirus (PoRV) strain, 134/04-15, identified in Italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known VP4 (P) genotypes. The aa sequence of the full-length VP4 protein of the PoRV strain 134/04-15 showed aa identity values ranging from 59.7% (bovine strain KK3, P811]) to 86.09% (porcine strain A46, P13]) with those of the remaining 25 P genotypes. Moreover, aa sequence analysis of the corresponding VP8* trypsin cleavage fragment revealed that the PoRV strain 134/04-15 shared low identity, ranging from 37.52% (bovine strain 993/83, P17]) to 73.6% (porcine strain MDR-13, P13]), with those of the remaining 25 P genotypes. Phylogenetic relationships showed that the VP4 of the PoRV strain 134/04-15 shares a common evolutionary origin with porcine P13] and lapine P22] rotavirus strains. Additional sequence analyses of the VP7, VP6, and NSP4 genes of the PoRV strain 134/04-15 revealed the highest VP7 aa identity (95.9%) to G5 porcine strains, a porcine-like VP6 within VP6 genogroup I, and a Wa-like (genotype B) NSP4, respectively. Altogether, these results indicate that the PoRV strain 134/04-15 should be considered as prototype of a new VP4 genotype, P26], and provide further evidence for the vast genetic and antigenic diversity of group A rotaviruses. |
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Keywords: | Rotavirus Diarrhea Pigs VP4 P genotype Genetic diversity |
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