| HPLC法测定复方金霉素甘油中盐酸金霉素的含量 |
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| 引用本文： | 焦志铁,张树倩. HPLC法测定复方金霉素甘油中盐酸金霉素的含量[J]. 中国药事, 2009, 23(11): 1115-1116,1119 |
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| 作者姓名： | 焦志铁 张树倩 |
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| 作者单位： | 江苏省扬州市第一人民医院,扬州,225001 |
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| 摘 要： | 目的建立HPLC法测定本院制剂复方金霉素甘油中盐酸金霉素的含量。方法采用Autima C18柱;流动相为甲醇-0.01mol.L-1NaH2PO4（以磷酸调节pH至3.3）（36∶64）;流速：1.0mL.min-1;检测波长：355nm。结果盐酸金霉素在85.56~513.36μg.mL-1范围内线性关系良好（r=0.9997）;平均回收率为100.23%,RSD为1.34%。结论本方法简便,快速,准确,重现性好,可用于复方金霉素甘油的质量控制。
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| 关 键 词： | 盐酸金霉素 复方金霉素甘油 HPLC |
Determination of Chlortetracycline Hydrochloride in Compound Chlortetracyctini Glycerin by HPLC |
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| Jiao Zhitie,Zhang Shuqian. Determination of Chlortetracycline Hydrochloride in Compound Chlortetracyctini Glycerin by HPLC[J]. Chinese Pharmaceutical Affairs, 2009, 23(11): 1115-1116,1119 |
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| Authors: | Jiao Zhitie Zhang Shuqian |
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| Affiliation: | Jiao Zhitie and Zhang Shuqian(The First People＇s Hospital of Yangzhou in Jiangsu Province, Yangzhou 225001) |
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| Abstract: | Objective To establish a method for determination of chlortetracycline hydrochloride in compound chlortetracyctini glycerin.Methods The method of HPLC was applied.The sample was separated on Autima C18 column with a mixture of methanol and 0.01mol·L-1 NaH2PO4 solution（36∶64） as the mobile phase.The UV detector wavelength was 355nm.Results The linearity was obtained within the range of chlortetracycline hydrochloride concentrations from 85.56μg·mL-1 to 513.36μg·mL-1,r=0.9997.The average recovery was 100.23%,, and RSD was 1.34%. Conclusions The method is simple, rapid, accurate and repeatable, which could be used to quality control of compound chlortetracyctini glycerin. |
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| Keywords: | HPLC |
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