维吾尔族妇女子宫颈癌中 p53 基因的表达和 HPV DNA 检测研究

应用Ｅ６特异性引物ＰＣＲ检测新疆维吾尔族妇女子宫颈癌活检组织标本中的ＨＰＶ６，１６和１８ＤＮＡ。并应用免疫组化研究ｐ５３基因的表达。结果显示，６５例标本中７例不适应用于ＰＣＲ，其余５８例中ＨＰＶ１６ＤＮＡ阳性４５例（７７．６％），而ＨＰＶ６ＤＮＡ和１８ＤＮＡ为阴性。免疫组化显示，６５例中ｐ５３表达阳性４９例（７５．４％）。在４５例ＨＰＶＤＮＡ阳性标本中，ｐ５３表达阳性３４例（７５．６％）；１３例ＨＰＶＤＮＡ阴性标本中，ｐ５３表达阳性１１例（８４．６％）。提示，ＨＰＶ阳性或阴性与ｐ５３表达之间无显著性差异（Ｐ＞０．０５）。

Immunostaining of the p53 Gene and Detection of Human Papillomavirus (HPV) in Cervical Carcinoma of Uygur Women

Cancer of the uterine cervix is one of the most common malignancies in women worldwide. Strong evidence supports that human papillomavirus (HPV) infection, especially HVP types 16 and 18, may be the role in the genesis of these tumors. HPV 16 and 18 both included two transforming gene products, the E6 and E7 protein. Action of these protein may be mediated by their interaction with a tumor suppresser gene. The p53 protein plays an important role in controlling proliferation of neoplastic and normal cells. We examined 65 cervical neoplasms from a population of women at particularly high risk for development of carvical cancer in Xinjiang. HPV DNA analysis using type-specific PCR for the detection of HPV type 6,16 and 18. Using immunostaining for the p53 protein overexpression. Besides, seven cases unavailable for PCR, in the remaining 58 cases, 45(77.6%) cases demonstrated only positive with HPV type 16, and no one case positive with HPV type 6 or 18. In addition, using immunostaining of the p53 protein showed 49 out of 65(75.4%) positive cases. In 45 HPV positive cases, 35(77.8%) showed p53 positive, and in 13 HPV negative cases, 10(76.9%) also showed p53 positive.There was no correlation with HPV status and the p53 protein overexpression being found.