七叶皂苷钠对大鼠脑缺血再灌注损伤SOD的影响

目的:观察七叶皂苷钠对大鼠脑缺血再灌注损伤中超氧化物歧化酶(SOD)的影响,探讨其治疗作用机制。方法:将试验大鼠随机分为假手术组、对照组、治疗组。用线栓法制作大鼠左侧大脑中动脉阻塞再灌注(MCAO/R)模型,应用七叶皂苷钠进行干预,采用免疫组化方法分别观察脑缺血再灌注6h、12h、1d、3d、7d和14d的SOD的表达以及藻蓝蛋白的干预作用。结果:对照组脑组织SOD有微弱表达,脑缺血再灌注后6h,皮质区和纹状体区SOD表达逐渐增强,于24h达高峰,之后逐渐升高,至7～14d仍高于假手术组。七叶皂苷钠对SOD变化趋势与对照组相似,与对照组同一时间点比较,SOD表达明显增强(P<0.01)。结论:脑缺血再灌注损伤后,七叶皂苷钠可能通过上调SOD的表达,对脑缺血再灌注损伤后神经细胞损伤具有保护作用。

Effect of sodium aescinate on changes of superoxide dismutase in blood after cerebral ischemic reperfusion injury in rats

Objective:To observe the effect of sodium aescinate on changes of superoxide dismutase(SOD),contend in blood after cerebral ischemic reperfusion injury in rats and to explore its mechanism on treatment. Methods:The model of middle cerebral artery occlusion reperfusion (MCAO/R) was established using the intraluminal filament occlusion with 52 healthy adult male Wistar rats, and treated by sodium aescinate. The apoptosis and the expression of SOD were determined by immunohistochemical staining to evaluate the effects of sodium aescinate on above indexes.Results:Sodium aescinate increased SOD activities.Conclusion:Sodium aescinate has the protective effect on cerebral ischemic reperfusion injury in rats and increasing SOD activitiy.