Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity |
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Authors: | Liao Mai-Jing Zhang Cheng Cheng Zhou Beiyan Zimonjic Drazen B Mani Sendurai A Kaba Megan Gifford Ann Reinhardt Ferenc Popescu Nicholas C Guo Wenjun Eaton Elinor Ng Lodish Harvey F Weinberg Robert A |
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Institution: | Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA. |
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Abstract: | The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of new cell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC. |
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