有机物对H2O2低温等离子灭菌效果影响的研究

目的通过有机物对H2O2等离子低温灭菌效果的影响,寻找内镜消毒灭菌中的薄弱环节,为进一步规范内镜消毒提供理论依据。方法选取造成医院感染源之一的大肠艾希菌为代表,取小牛血清作为有机物,并取25%、50%的小牛血清浓度为可变因素,研究有机物对H2O2等离子低温灭菌效果的影响。结果无小牛血清存在的情况下,H2O2等离子低温灭菌系统作用5 min后杀灭率可以达到100%,而有25%和50%小牛血清存在时,对杀菌效果有影响。结论有机物对H2O2等离子低温灭菌系统灭菌效果有显著的影响,在有机物存在时其杀菌作用明显减弱,且浓度越高影响越大。因此,器械灭菌前必须彻底清洗干净。

Study on the disinfection effect of H2O2 low-temperature plasma affected by organic matter

Objective To supply academic proof of standardizing endoscope sterilization,and to seek for the substantial process of endoscope sterilization. Method Study the influence of organism(calf serum) on the H_2O_2 low-temperature plasma and study the influence of organic matter by using concentration of 25% and 50% calf serum as alterable factors.Result The results showed the kill ratio of the low-temperature plasma was 100% after 5 min without organism.But the kill ratio was 99.84% after 5 min with 25% organism,and the treated time was longer,the kill ratio was higher.After 20 min,the kill ratio was 100%.With 50% organism,the kill ratio was 99.46% after 5mins and 100% after 30 min.Conclusion Organism resulted in a significantly weaker disinfection action of low-temperature plasma,and the kill ratio was associated with the treated time.Effective cleaning is an essential pre-requisite for appliance sterilization.