新型化合物FK106诱导白血病细胞凋亡的作用研究

目的 研究新型化合物FK106对白血病细胞增殖的抑制作用及其可能的机制。方法 采用噻唑蓝（MTT）法检测FK106对白血病MV4-11细胞存活率的影响，采用PI染色流式细胞仪检测FK106对细胞周期的影响，通过Annexin V-FITC/PI 双染流式细胞仪检测FK106对细胞凋亡的影响，采用Western blot检测凋亡相关蛋白表达的影响。结果 FK106对白血病细胞MV4-11的增殖具有抑制作用，且在0.1~1.0 mmol·L^-1浓度范围内，对白血病MV4-11的抑制率具有浓度、时间依赖关系；FK106能阻滞 MV4-11细胞G₀/G₁期；随着FK106浓度的增加，MV4-11细胞凋亡率呈现增高的趋势，并呈剂量依赖性；FK106可以抑制 Bcl-2和Fas mRNA的表达，同时Bax和Caspase-3 mRNA表达明显增加，以上均呈依赖性。结论 FK106能抑制人白血病细胞MV4-11细胞的增殖,并诱导其发生凋亡,可能通过Bcl-2和Fas途径诱导MV4-11细胞凋亡，具有较好的临床应用前景。

Study on the effect of new compound FK106 on apoptosis induced by leukemia cells

Objective To study the inhibitory effect of new compound FK106 on leukemia cell proliferation and its possible mechanism.Methods The effects of FK106 on the cell survival rate of leukemia MV4-11 were detected by MTT, the effects of FK106 on cell cycle were detected by PI staining flow cytometry, and the effects of FK106 on apoptosis were detected by Annexus V-FITC/PI double staining flow cytometry. the expression of apoptosis related proteins were detected by Western blot.Results FK106 had inhibitory effect on the proliferation of leukemia cell MV4-11, and the inhibition rate had concentration and time-dependent within the concentration of 0.1~1.0 mmol·L^-1 of FK106. FK106 can block the MV4-11 cell G0/G1 phase.The apoptosis rate of MV4-11 cells had an increasing trend with the increase of FK106 concentration, and the apoptosis rate of cells was dose-dependent. FK106 inhibited the expression of Bcl-2 and Fas mRNA, while Bax and Caspase-3 mRNA expression increased significantly, and the expression of these protein were dose-dependent.Conclusion FK106 can inhibit the proliferation of MV4-11 cells of human leukemia cells and induce apoptosis. It may induce MV4-11 cells apoptosis through Bcl-2 and Fas pathways, which has a good clinical application prospect.