山奈酚对白假丝酵母生物被膜抑制作用的研究

目的 研究山奈酚抗白假丝酵母生物被膜的作用及其可能机制。方法 测定山奈酚对白假丝酵母处于形成过程中的生物被膜和成熟生物被膜代谢活性的影响；测定山奈酚对生物被膜基质产生水平的影响；显微镜下观察山奈酚对菌丝形成的抑制作用；水-烃两相分离法测定山奈酚对白假丝酵母细胞表面疏水性的影响；实时定量RT-PCR法测定山奈酚对生物被膜形成相关基因表达的影响。结果 山奈酚抑制白假丝酵母生物被膜形成，且呈剂量依赖性，同时具有抗成熟生物被膜作用，显著降低生物被膜基质含量；与对照组相比，山奈酚明显抑制白假丝酵母菌丝形成并降低其细胞表面疏水性；经山奈酚处理的白假丝酵母生物被膜形成相关基因BCR1、NRG1和TUP1的表达升高，同时HWP1、EFG1、CPH1、ALS1、ALS3和CSH1的表达下降。结论 山奈酚具有抗白假丝酵母生物被膜活性，其机制与抑制菌丝形成及降低其细胞表面疏水性相关。

Study on the antibiofilm activity of kaempferol in Candida albicans

Objective To study the action of kaempferol (KAE) against Candida albicans biofilms and explore the potential mechanisms.Methods Biofilm metabolic activity assay was used to investigate the action of KAE against C. albicans biofilm formation as well as mature biofilm. The inhibition of KAE in hyphal formation was examined by microscope. The water-hydrocarbon two-phase separation assay was used to test the effect of KAE on the cell surface hydrophobicity of C. albicans. The mRNA expression of the genes involved in biofilm formation was determined by real time RT-PCR.Results KAE showed inhibition effect on C. albicans biofilm formation in a dose-dependent manner. Moreover, KAE inhibited mature biofilm. The biomass of biofilm was reduced upon KAE treatment. KAE inhibited hyphal formation and reduced the cell surface hydrophobicity of C. albicans. In the presence of KAE, the mRNA expression of the genes involved in biofilm formation was changed, with the up-regulation of BCR1,NRG1,TUP1 and down-regulation of HWP1,EFG1,CPH1,ALS1,ALS3 and CSH1.Conclusion KAE showed antifungal activity against C. albicans biofilm. The mechanisms may relate to the inhibition of hyphal formation and reduction of cell surface hydrophobicity.