环状RNA circPRDM5在食管鳞状细胞癌中的表达和功能研究

［摘要］ 目的 探索环状RNA circPRDM5在食管鳞状细胞癌（ESCC）细胞和组织中的表达水平，及其对ESCC细胞功能的影响。方法 qRT?PCR检测circPRDM5在正常食管上皮细胞（HEEC）、ESCC细胞系、和44例ESCC组织及对应的癌旁正常组织中的相对表达水平；Transwell实验和CCK8实验分别检测siRNA沉默circPRDM5后，对细胞迁移侵袭和增殖功能的影响；构建裸鼠成瘤模型，检测circPRDM5对ESCC细胞在动物体内成瘤能力的影响。结果 与HEEC相比，circPRDM5在ESCC细胞系KYSE150、ECA109中高表达（P<0.05），且在ESCC组织中表达（2.90±0.18）较癌旁正常组织（2.01±0.15）表达高（P<0.001）。Transwell实验和CCK8实验证明，沉默circPRDM5能明显抑制ESCC细胞的迁移侵袭和增殖能力（P<0.05）；裸鼠成瘤实验结果表明，沉默circPRDM5后，ESCC细胞的成瘤体积和重量较对照组明显下降（P<0.01）。结论 circPRDM5在ESCC的细胞和组织中高表达。在ESCC细胞中沉默circPRDM5能降低ESCC细胞的增殖、迁移侵袭、和在动物体内的成瘤能力。提示circPRDM5可能成为ESCC治疗的一个新靶点。

Expression and function of circular RNA circPRDM5 in esophageal squamous cell carcinoma

［Abstract］ Objective To investigate the expression level of circular RNA circPRDM5 in esophageal squamous cell carcinoma （ESCC） cells and tissues， and its effect on ESCC cell function. Methods qRT?PCR was used to detect the relative expression of circPRDM5 in normal esophageal epithelial cells （HEEC）， ESCC cell line， and 44 ESCC tissues and corresponding adjacent normal tissues； Transwell and CCK8 assays were performed to detect the effects on cell migration， invasion and proliferation after siRNA silencing circPRDM5； a nude mouse model was established to examine the effect of circPRDM5 on the tumorigenic ability of ESCC cells in animals. Results Compared with HEEC， circPRDM5 was highly expressed in ESCC cell lines KYSE150 and ECA109 （P<0.05）， and the expression in ESCC tissues （2.90±0.18） was higher than that in adjacent normal tissues （2.01±0.15）（P<0.001）； Transwell assay and CCK8 assay demonstrated that silencing of circPRDM5 significantly inhibited the migration， invasion and proliferation of ESCC cells （P<0.05）. The results of tumor formation in nude mice showed that the volume and weight of ESCC cells after silencing circPRDM5 were significantly lower than those of the control group （P<0.01）. Conclusion circPRDM5 is highly expressed in the cells and tissues of ESCC. Silencing circPRDM5 in ESCC cells reduced the proliferation， migration and invasion of ESCC cells， and the vivo tumorigenesis abilities. It is suggested that circPRDM5 may become a new target for ESCC treatment.