| HPLC法测定叶黄素的平衡溶解度及表观油水分配系数 |
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| 引用本文： | 朱溶月,常道潇,隋宏,孔银弟,王文苹. HPLC法测定叶黄素的平衡溶解度及表观油水分配系数[J]. 药学研究, 2018, 37(7): 388-390,412 |
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| 作者姓名： | 朱溶月 常道潇 隋宏 孔银弟 王文苹 |
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| 作者单位： | 1.宁夏医科大学药学院，宁夏 银川 750004,1.宁夏医科大学药学院，宁夏 银川 750004,1.宁夏医科大学药学院，宁夏 银川 750004,1.宁夏医科大学药学院，宁夏 银川 750004,1.宁夏医科大学药学院，宁夏 银川 750004;2.宁夏回药现代化工程技术研究中心，宁夏 银川 750004；3.回医药现代化省部共建教育部重点实验室，宁夏 银川 750004 |
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| 基金项目： | 宁夏高等学校科学研究项目（No.NGY2015082）；宁夏医科大学科学研究基金（No.XY2017008） |
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| 摘 要： | 目的 测定叶黄素在不同溶剂中的平衡溶解度及表观油水分配系数。方法 建立高效液相色谱法用于测定叶黄素含量，采用摇瓶法考察叶黄素在多种有机溶剂及不同pH值缓冲液中的平衡溶解度，并测定其表观油水分配系数。结果 叶黄素几乎不溶于水及缓冲液，在有机溶剂中溶解度有不同程度提高，其中在四氢呋喃中的溶解度最高（66 mg·mL-1）；其表观油水分配系数为1.03。结论 叶黄素在多数溶剂中的溶解度均较低，可通过提高溶解度改善溶出和吸收。
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| 关 键 词： | 叶黄素；高效液相色谱法；平衡溶解度；表观油水分配系数 |
Determination of equilibrium solubility and octanol-water partition coefficient of lutein by HPLC |
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| ZHU Rongyue,CHANG Daoxiao,SUI Hong,KONG Yindi and WANG Wenping. Determination of equilibrium solubility and octanol-water partition coefficient of lutein by HPLC[J]. Journal of Pharmaceutical Research, 2018, 37(7): 388-390,412 |
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| Authors: | ZHU Rongyue CHANG Daoxiao SUI Hong KONG Yindi WANG Wenping |
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| Affiliation: | 1.School of Pharmacy, Ningxia Medical University,Yinchuan 750004,China;2.Ningxia Engineering & Technology Research Center For Modernization of Hui Medicine,Yinchuan 750004,China;3.Key Lab of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004,China,1.School of Pharmacy, Ningxia Medical University,Yinchuan 750004,China;2.Ningxia Engineering & Technology Research Center For Modernization of Hui Medicine,Yinchuan 750004,China;3.Key Lab of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004,China,1.School of Pharmacy, Ningxia Medical University,Yinchuan 750004,China;2.Ningxia Engineering & Technology Research Center For Modernization of Hui Medicine,Yinchuan 750004,China;3.Key Lab of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004,China,1.School of Pharmacy, Ningxia Medical University,Yinchuan 750004,China;2.Ningxia Engineering & Technology Research Center For Modernization of Hui Medicine,Yinchuan 750004,China;3.Key Lab of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004,China and 1.School of Pharmacy, Ningxia Medical University,Yinchuan 750004,China;2.Ningxia Engineering & Technology Research Center For Modernization of Hui Medicine,Yinchuan 750004,China;3.Key Lab of Hui Ethnic Medicine Modernization,Ministry of Education,Yinchuan 750004,China |
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| Abstract: | Objective To determine the equilibrium solubility of lutein in different solvents and their partition coefficients.Methods The high performance liquid chromatography (HPLC) method was established and used to detect the equilibrium solubility of lutein in different solvents. Their octanol-water partition coefficients were determined by shaking flask combined with HPLC.Results The solubility of lutein in water and buffer solution was very low, and its solubility in organic solvent increased to some extent. Among them, the solubility of lutein in tetrahydrofuran was highest (66 mg·mL-1). The octanol-water partition coefficient was 1.03.Conclusion The solubility of lutein was lower in most solvents and the dissolution and absorption could be improved by increasing solubility. |
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| Keywords: | Lutein HPLC Equilibrium solubility Octanol-water partition coefficients |
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