淫羊藿苷纳米微粒对大鼠成骨细胞成熟与矿化的影响

目的观察淫羊藿苷纳米微粒(ICA-NS)对出生48 h内的大鼠乳鼠颅骨成骨细胞成熟与矿化的作用。方法显微镜观察淫羊藿苷纳米微粒对成骨细胞形态的影响; Hoechst 3342/PI双染色法检测淫羊藿苷纳米微粒对成骨细胞的毒性反应;碱性磷酸酶检测试剂盒检测成骨细胞中碱性磷酸酶(ALP)活性;茜苏红染料对淫羊藿苷纳米微粒处理过的细胞进行染色,观察钙化结节的数量和面积;检测成骨细胞中与成骨相关的特异性蛋白表达情况。结果与淫羊藿苷组相比,淫羊藿苷纳米微粒组细胞形态无明显变化,且Hoechst 3342/PI双染色法结果进一步显示淫羊藿苷纳米微粒对成骨细胞无明显毒副作用;碱性磷酸酶活性测定结果显示淫羊藿苷纳米微粒显著提高了成骨细胞中碱性磷酸酶水平,而且淫羊藿苷纳米微粒组茜苏红钙化结节染色面积明显增大,颜色显著加深,成骨性相关蛋白的表达量也显著高于对照组。结论淫羊藿苷纳米微粒能显著促进成骨细胞的矿化与成熟,且对成骨细胞无明显毒副作用。

Effect of icariin nanoparticles on osteoblast maturation and mineralization in rats

Objective To observe the effect of icariin nanoparticles on the maturation and mineralization of rat osteoblasts in the 48-hour period of birth. Methods The effect of icariin nanoparticles on the morphology of osteoblasts was observed with a microscope. The toxicity of icariin nanoparticles to osteoblasts was detected with Hoechst 3342/PI double staining. Alkaline phosphatase activity in osteoblasts was detected with a alkaline phosphatase detection kit. The saponin dye was used to stain the cells treated with icariin nanoparticles to observe the number and area of calcified nodules. The specific protein expression related to osteogenesis in osteoblasts was detected. Results Compared with those in the icariin group, the osteoblasts treated with icariin nanoparticles showed no significant changes in morphology. Hoechst 3342/PI double staining results further showed that there were no obvious toxic and side effects of icariin nanoparticles on osteoblasts. Icariin nanoparticles significantly increased the alkaline phosphatase activity in osteoblasts. The staining area of sorghum red calcified nodules increased in osteoblasts treated with icariin nanoparticles and the color was significantly deeper. The expression levels of osteogenic related proteins were also significantly higher than those in the control group. Conclusion Icariin nanoparticles significantly promotes the mineralization and maturation of osteoblasts, and has no obvious side effects on osteoblasts.