2型糖尿病肾病患者肾组织中 STOML2的表达及作用 |
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引用本文: | 陈浩雄,傅君舟,何凤,刘日光.2型糖尿病肾病患者肾组织中 STOML2的表达及作用[J].广州医药,2016(4):42-45. |
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作者姓名: | 陈浩雄 傅君舟 何凤 刘日光 |
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作者单位: | 广州市第一人民医院 肾内科 广州 510180 |
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基金项目: | 广州市医药卫生科技引导项目(20151 A010019) |
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摘 要: | 目的:探讨2型糖尿病肾病(DN)患者肾组织中 STOML2的表达及作用。方法免疫组化检测临床2型糖尿病肾病患者肾组织 STOML2的表达及定位,采用慢病毒转染方法建立稳定过表达 STOML2的 HK-2细胞系,并应用 Western blot 检测肾小管上皮细胞钙粘蛋白(E-cadherin)、Fibronectin 和 STOML2的表达。结果 STOML2在 DN患者肾组织的肾小管上皮细胞胞浆中表达明显升高。在高糖刺激 HK-2细胞建立的 EMT 模型中,STOML2呈时间依赖表达上调。STOML2稳定高表达时,E-cadherin 表达下调,而 Fibronectin 明显上调,即能促进肾小管上皮细胞发生EMT。结论 STOML2可能通过促进肾小管上皮细胞向间充质细胞分化,进而参与糖尿病肾病肾脏纤维化的发生发展。
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关 键 词: | 糖尿病肾病 上皮细胞-间质转化 纤维化 STOML2 |
The role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy |
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Abstract: | Objective To investigate the expression and role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy. Methods To detect he expression and localization of STOML2 in clinical renal tissue in patients with type 2 dia-betic nephropathy by immunohistochemistry,and use lentiviral transfection method to establish a stable cell line of over-express-ing STOML2,lastly apply western blot to detect the expression of E-cadherin,Fibronectin and STOML2 in renal tubular epithelial cells. Results STOML2 was significantly increased in renal tubular epithelial cytoplasm of patients with DN.In the EMT model of HK-2 cells stimulated by high glucose,STOML2 was increased in a time dependent.Overexpression of STOML2 led to E-cadherin down-regulated,while Fibronectin up-regulated,which promoted the occurrence of EMT in renal tubular epithelial cells. Conclusion STOML2 may be involved in the development and progression of renal fibrosis in diabetic nephropathy by mediating epithelial-mesenchymal transition of renal tubular epithelial cells. |
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Keywords: | Diabetic nephropathy Epithelial-mesenchymal transition Fibrosis STOML2 |
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