2型糖尿病肾病患者肾组织中 STOML2的表达及作用

目的：探讨2型糖尿病肾病（DN）患者肾组织中 STOML2的表达及作用。方法免疫组化检测临床2型糖尿病肾病患者肾组织 STOML2的表达及定位，采用慢病毒转染方法建立稳定过表达 STOML2的 HK-2细胞系，并应用 Western blot 检测肾小管上皮细胞钙粘蛋白（E-cadherin）、Fibronectin 和 STOML2的表达。结果 STOML2在 DN患者肾组织的肾小管上皮细胞胞浆中表达明显升高。在高糖刺激 HK-2细胞建立的 EMT 模型中，STOML2呈时间依赖表达上调。STOML2稳定高表达时，E-cadherin 表达下调，而 Fibronectin 明显上调，即能促进肾小管上皮细胞发生EMT。结论 STOML2可能通过促进肾小管上皮细胞向间充质细胞分化，进而参与糖尿病肾病肾脏纤维化的发生发展。

The role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy

Objective To investigate the expression and role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy. Methods To detect he expression and localization of STOML2 in clinical renal tissue in patients with type 2 dia-betic nephropathy by immunohistochemistry,and use lentiviral transfection method to establish a stable cell line of over-express-ing STOML2,lastly apply western blot to detect the expression of E-cadherin,Fibronectin and STOML2 in renal tubular epithelial cells. Results STOML2 was significantly increased in renal tubular epithelial cytoplasm of patients with DN.In the EMT model of HK-2 cells stimulated by high glucose,STOML2 was increased in a time dependent.Overexpression of STOML2 led to E-cadherin down-regulated,while Fibronectin up-regulated,which promoted the occurrence of EMT in renal tubular epithelial cells. Conclusion STOML2 may be involved in the development and progression of renal fibrosis in diabetic nephropathy by mediating epithelial-mesenchymal transition of renal tubular epithelial cells.