Fmr1基因敲除小鼠悬尾实验的观察

目的：对不同周龄的 KO 小鼠与 WT 小鼠进行悬尾实验进行观察，探讨 KO 小鼠与 WT 小鼠的行为差别。方法采用健康的试验动物180只分两组：①KO 组（4、6、8周龄，各周龄30只，雌雄各半，共90只）②WT 组（4、6、8周龄，各周龄30只，雌雄各半，共90只）；通过悬尾实验观察性别，年龄对不动时间的影响。结果同龄 KO 雌性小鼠比雄性小鼠的静止时间差别不大；随着年龄增大，静止时间增长。同龄同性别的 KO 鼠比 WT 鼠的不动时间长。P ＜0．05；同龄雄性小鼠比雌性小鼠的不动时间短；随年龄增长各种系小鼠不动时间增长，KO 鼠的不动时间比 WT 鼠长，P ＜0．05。结论 KO 小鼠存在抑郁行为表型。

Observation of tail suspension test in Fmr1 gene knockout mice

Objective To observe tail suspension test in Fmr1 gene knockout mice and to explore whether there are differences in mobility of KO and WT mice. Methods 1 80 test mice were divided into two groups:① KO group (4,6,8 weeks old,each age group of mice is 30,male and female in half,a total of 90)② WT group (4,6,8 weeks old,each group of mice is 30,male and female on half,a total of 90).Through forced swimming test and tail suspension test to observe gender, age effect on immobility time. Results With the same age of the same sex,the KO mice’s immobility time was longer than WT mice’s.P <0.05.With the same age,the male mice’s immobility time was shorter than female mice’s.With the age in-crease,the immobility time of KO mice was longer than WT mice.P <0.05. Conclusion Fmr1 gene knockout mice have anxiety and depressive behavior.