CGRP和NGF对脑局部缺血再灌注大鼠脑组织神经元凋亡及PKCmRNA表达的调节作用

目的研究大鼠脑缺血再灌注后蛋白激酶C信使RNA(PKCmRNA)的表达,探讨降钙素基因相关肽(CGRP)和神经生长因子(NGF)对脑组织缺血再灌注的保护作用及机制。方法采用颈动脉负压分流法制作大鼠脑缺血再灌注模型,采用TUNEL法、原位杂交方法及显微图像分析检测海马及皮质内神经元凋亡和PKCmRNA的表达。结果大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数及PKCmRNA反应产物较正常组增多(P<0.05),而注射CGRP或NGF后神经元凋亡数及PKCmRNA反应产物明显低于缺血再灌注组(P<0.01),二者联合应用效果更加显著(P<0.05)。结论CGRP和NGF抑制缺血神经元凋亡,参与PKCmRNA的调节,二者对缺血神经元有协同修复作用。

The effect of CGRP and NGF on the expression of PKC during regional cerebral ischemia and reperfusion in rats

Objective To investigate the expression of protein kinase C mRNA(PKCmRNA) in hippocampus after whole cerebral ischemia and reperfusion in rats, explore the protective effects and mechanism of calcitonin gene-related peptide(CGRP) and nerve growth factor(NGF) on brain tissue. Methods An novel model of whole cerebral ischemia and reperfusion induced by carotid artery negative pressure shunt in rats; the expression of PKCmRNA and apoptosis cell in hippocampus and cortex was detected with In Situ Hybridization, TUNEL method and analyzed by microimage system. Results The expression of PKCmRNA and apoptosis cell in hippocampus and cortex was increased after cerebral ischemia and reperfusion(P<0.05); compared with this, the expression of PKCmRNA reaction positive cells and apoptosis cell in CGRP or NGF group were lower (P<0.01); the combined usage of CGRP and NGF enhanced such effects(P<0.05). Conclusion The results indicate that CGRP and NGF depress cell apoptosis ,participate in the regulation of expression of PKCmRNA in ischemic neurons,and they may cooperate with each other.