冠心病患者对氧磷酶基因192位Gln-Arg多态性的研究

目的　探讨冠心病 (CHD)患者血脂改变是否与对氧磷酶 (PON)基因 192位 Gln- Arg变异有关。方法　应用多聚酶链反应 (PCR)对成都地区汉族 118例冠心病患者及 12 8例正常对照者 PON基因酶切位点的限制性片段长度多态性 (RFL P)进行研究。血脂用酶法测定。结果　 CHD患者和正常对照组 PON基因均以 QR基因型为主 ,其频率分别为 0 .5 78和 0 .5 2 5。正常对照组和 CHD组 PON基因 R等位基因频率分别为 0 .5 2和 0 .5 0 ,无显著差异 (P>0 .0 5 )。中国人 PON基因 R等位基因频率显著高于白种人及印度人 (P<0 .0 1) ,而低于日本人及中国台北人(P<0 .0 1) ,存在种族差异。结论　未发现 PON1基因 192位 Gln- Arg多态性与中国人 CHD有关联

The Paraoxonase Gln-Arg 192 Polymorphism in Patients with Co ronary Heart Disease in Chinese Population

Objective To investigate the 192 Gln Arg polymorphism of paraoxonase (PON) gene and its relationship with serum lipids levels in patients with coronary heart disease in Chinese population. Methods The genotype and allele frequency of paraoxonase gene 192 Gln Arg polymorphism was assayed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Serum lipids were measured by enzymatic kits in 118 CHD patients and 128 healthy subjects whose fasting serum TG levels were <1.82 mmol/L and TC levels <6.2 mmol/L from a population of Chinese Han nationality in Chengdu area. Results Both in CHD group and control group, the QR genotype of PON gene was the major one, and its frequencies were 0.578 and 0.525 respectively. No differences were found in PON gene Gln Arg polymophism in the CHD group when compared with the control group. The frequency of R allele of PON gene in Chinese was significant higher than that in European and American Caucasians (0.51 vs. 0.26~0.31, P< 0.01) and Indian (0.51 vs. 0.17, P< 0.01), and significantly lower than that in Japanese (0.51 vs. 0.65, P< 0.01). Conclusion These results suggested that the Gln Arg192 polymorphism of the PON1 gene was not associated with coronary heart disease in Chinese population.