| 新型二聚体突变荧光引物定量检测沙眼衣原体 |
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| 引用本文： | 夏乾峰,覃西,钱士匀,涂植光. 新型二聚体突变荧光引物定量检测沙眼衣原体[J]. 临床检验杂志, 2011, 29(4): 241-243 |
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| 作者姓名： | 夏乾峰 覃西 钱士匀 涂植光 |
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| 作者单位： | 重庆医科大学医学检验系，重庆400016;海南医学院热带医学与检验医学院，海口570102;海南医学院热带医学与检验医学院，海口570102;重庆医科大学医学检验系，重庆400016 |
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| 基金项目： | 国家自然科学基金资助项目（30872417）；海南省教育厅高校科学研究项目（Hj201020）。 |
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| 摘 要： | 目的 以二聚体突变荧光引物技术为基础建立对沙眼衣原体进行定量检测的新方法.方法 以沙眼衣原体主要外膜蛋白(MOMP)基因构建重组质粒作为DNA标准品,设计二聚体突变荧光引物,优化定量PCR体系并进行性能评价;同时对148例临床生殖道标本进行检测.结果 建立的二聚体突变荧光引物的定量PCR方法,其线性范围为101～109...
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| 关 键 词： | 沙眼衣原体 实时定量PCR 二聚体突变荧光引物 |
| 收稿时间： | 2010-12-04 |
| 修稿时间： | 2011-03-14 |
A novel quantitative real-time PCR assay using self reporting duplex mutation primers for detection of Chlamydia trachomatis |
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| XIA Qian-feng,QIN Xi,QIAN Shi-yun,TU Zhi-guang. A novel quantitative real-time PCR assay using self reporting duplex mutation primers for detection of Chlamydia trachomatis[J]. Chinese Journal of Clinical Laboratory Science, 2011, 29(4): 241-243 |
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| Authors: | XIA Qian-feng QIN Xi QIAN Shi-yun TU Zhi-guang |
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| Affiliation: | XIA Qian-feng1,QIN Xi2,QIAN Shi-yun2,TU Zhi-guang1(1.Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,2.School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou 571101,Hainan,China) |
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| Abstract: | Objective To establish a novel real-time fluorescence PCR method to detect Chlamydia trachomatis(CT) using self-reporting duplex mutation primers.Methods The recombinant vector was constructed with major outer membrane protein(MOMP) gene of CT and was used as the standard template.The self-reporting duplex mutation primers were designed according to the cloned gene sequence.The quantitative PCR reaction system was optimized and the experimental performance of real-time PCR was evaluated.A total of 148 clini... |
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| Keywords: | Chlamydia trachomatis real-time quantitative PCR self-reporting duplex mutation primer |
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